This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

2l8k

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 03:41, 10 September 2015

NMR Structure of the Arterivirus nonstructural protein 7 alpha (nsp7 alpha)

Structural highlights

2l8k is a 1 chain structure with sequence from Eav. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	rep, 1a-1b (EAV)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum

Function

[RPOA_EAVBU] The replicase polyprotein 1ab is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.^[1] Nsp1 is essential for viral subgenomic mRNA synthesis.^[2] Nsp2 cysteine proteinase which cleaves the nsp2/nsp3 site in the polyprotein. Also displays deubiquitinating and deISGylase activities. The deubiquitinating activity cleaves both ubiquitinated and ISGylated products and may therefore regulate ubiquitin and ISG15 dependent host innate immunity.^[3] The 3C-like serine proteinase chain is responsible for the majority of cleavages as it cleaves the C-terminus of the polyprotein.^[4] The helicase chain, which contains a zinc finger structure, displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity.^[5]

Publication Abstract from PubMed

Arterivirus replicase polyproteins are cleaved into at least 13 mature nonstructural proteins (nsps), and in particular the nsp5-to-nsp8 region is subject to a complex processing cascade. The function of the largest subunit from this region, nsp7, which is further cleaved into nsp7alpha and nsp7beta, is unknown. Using nuclear magnetic resonance (NMR) spectroscopy, we determined the solution structure of nsp7alpha of equine arteritis virus, revealing an interesting unique fold for this protein but thereby providing little clue to its possible functions. Nevertheless, structure-based reverse genetics studies established the importance of nsp7/nsp7alpha for viral RNA synthesis, thus providing a basis for future studies.

Structure and Genetic Analysis of the Arterivirus Nonstructural Protein 7{alpha}.,Manolaridis I, Gaudin C, Posthuma CC, Zevenhoven-Dobbe JC, Imbert I, Canard B, Kelly G, Tucker PA, Conte MR, Snijder EJ J Virol. 2011 Jul;85(14):7449-53. Epub 2011 May 11. PMID:21561912^[6]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Frias-Staheli N, Giannakopoulos NV, Kikkert M, Taylor SL, Bridgen A, Paragas J, Richt JA, Rowland RR, Schmaljohn CS, Lenschow DJ, Snijder EJ, Garcia-Sastre A, Virgin HW 4th. Ovarian tumor domain-containing viral proteases evade ubiquitin- and ISG15-dependent innate immune responses. Cell Host Microbe. 2007 Dec 13;2(6):404-16. PMID:18078692 doi:http://dx.doi.org/10.1016/j.chom.2007.09.014
↑ Frias-Staheli N, Giannakopoulos NV, Kikkert M, Taylor SL, Bridgen A, Paragas J, Richt JA, Rowland RR, Schmaljohn CS, Lenschow DJ, Snijder EJ, Garcia-Sastre A, Virgin HW 4th. Ovarian tumor domain-containing viral proteases evade ubiquitin- and ISG15-dependent innate immune responses. Cell Host Microbe. 2007 Dec 13;2(6):404-16. PMID:18078692 doi:http://dx.doi.org/10.1016/j.chom.2007.09.014
↑ Frias-Staheli N, Giannakopoulos NV, Kikkert M, Taylor SL, Bridgen A, Paragas J, Richt JA, Rowland RR, Schmaljohn CS, Lenschow DJ, Snijder EJ, Garcia-Sastre A, Virgin HW 4th. Ovarian tumor domain-containing viral proteases evade ubiquitin- and ISG15-dependent innate immune responses. Cell Host Microbe. 2007 Dec 13;2(6):404-16. PMID:18078692 doi:http://dx.doi.org/10.1016/j.chom.2007.09.014
↑ Frias-Staheli N, Giannakopoulos NV, Kikkert M, Taylor SL, Bridgen A, Paragas J, Richt JA, Rowland RR, Schmaljohn CS, Lenschow DJ, Snijder EJ, Garcia-Sastre A, Virgin HW 4th. Ovarian tumor domain-containing viral proteases evade ubiquitin- and ISG15-dependent innate immune responses. Cell Host Microbe. 2007 Dec 13;2(6):404-16. PMID:18078692 doi:http://dx.doi.org/10.1016/j.chom.2007.09.014
↑ Frias-Staheli N, Giannakopoulos NV, Kikkert M, Taylor SL, Bridgen A, Paragas J, Richt JA, Rowland RR, Schmaljohn CS, Lenschow DJ, Snijder EJ, Garcia-Sastre A, Virgin HW 4th. Ovarian tumor domain-containing viral proteases evade ubiquitin- and ISG15-dependent innate immune responses. Cell Host Microbe. 2007 Dec 13;2(6):404-16. PMID:18078692 doi:http://dx.doi.org/10.1016/j.chom.2007.09.014
↑ Manolaridis I, Gaudin C, Posthuma CC, Zevenhoven-Dobbe JC, Imbert I, Canard B, Kelly G, Tucker PA, Conte MR, Snijder EJ. Structure and Genetic Analysis of the Arterivirus Nonstructural Protein 7{alpha}. J Virol. 2011 Jul;85(14):7449-53. Epub 2011 May 11. PMID:21561912 doi:10.1128/JVI.00255-11

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2l8k"

@@ Line 2: / Line 2: @@
 <StructureSection load='2l8k' size='340' side='right' caption='[[2l8k]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2l8k]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Equine_arteritis_virus Equine arteritis virus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L8K FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2l8k]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Eav Eav]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L8K FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rep, 1a-1b ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11047 Equine arteritis virus])</td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rep, 1a-1b ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=299386 EAV])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l8k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2l8k RCSB], [http://www.ebi.ac.uk/pdbsum/2l8k PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l8k OCA], [http://pdbe.org/2l8k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2l8k RCSB], [http://www.ebi.ac.uk/pdbsum/2l8k PDBsum]</span></td></tr>
 </table>
 == Function ==
@@ Line 16: / Line 16: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 2l8k" style="background-color:#fffaf0;"></div>
 ==See Also==
@@ Line 23: / Line 24: @@
 __TOC__
 </StructureSection>
-[[Category: Equine arteritis virus]]
+[[Category: Eav]]
 [[Category: Conte, M R]]
 [[Category: Gaudin, C]]

2l8k

From Proteopedia

Revision as of 03:41, 10 September 2015

NMR Structure of the Arterivirus nonstructural protein 7 alpha (nsp7 alpha)

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox