1wlj

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|PDB= 1wlj |SIZE=350|CAPTION= <scene name='initialview01'>1wlj</scene>, resolution 1.9&Aring;
|PDB= 1wlj |SIZE=350|CAPTION= <scene name='initialview01'>1wlj</scene>, resolution 1.9&Aring;
|SITE=
|SITE=
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|LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene> and <scene name='pdbligand=U5P:URIDINE-5'-MONOPHOSPHATE'>U5P</scene>
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|LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene> and <scene name='pdbligand=U5P:URIDINE-5&#39;-MONOPHOSPHATE'>U5P</scene>
|ACTIVITY=
|ACTIVITY=
|GENE=
|GENE=
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[[Category: exoribonuclease]]
[[Category: exoribonuclease]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:58:31 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 14:07:26 2008''

Revision as of 12:07, 23 March 2008

Image:1wlj.jpg


PDB ID 1wlj

Drag the structure with the mouse to rotate
, resolution 1.9Å
Ligands: , and
Coordinates: save as pdb, mmCIF, xml



human ISG20


Overview

ISG20 is an interferon-induced antiviral exoribonuclease that acts on single-stranded RNA and also has minor activity towards single-stranded DNA. It belongs to the DEDDh group of RNases of the DEDD exonuclease superfamily. We have solved the crystal structure of human ISG20 complexed with two Mn2+ ions and uridine 5'-monophosphate (UMP) at 1.9 A resolution. Its structure, including that of the active site, is very similar to those of the corresponding domains of two DEDDh-group DNases, the epsilon subunit of Escherichia coli DNA polymerase III and E. coli exonuclease I, strongly suggesting that its catalytic mechanism is identical to that of the two DNases. However, ISG20 also has distinctive residues, Met14 and Arg53, to accommodate hydrogen bonds with the 2'-OH group of the UMP ribose, and these residues may be responsible for the preference of ISG20 for RNA substrates.

About this Structure

1WLJ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of human ISG20, an interferon-induced antiviral ribonuclease., Horio T, Murai M, Inoue T, Hamasaki T, Tanaka T, Ohgi T, FEBS Lett. 2004 Nov 5;577(1-2):111-6. PMID:15527770

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