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1yue

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Revision as of 09:36, 10 September 2015

Bacteriophage T4 capsid vertex protein gp24

Structural highlights

1yue is a 1 chain structure with sequence from Bpt4. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
NonStd Res:
Gene:	24 (BPT4)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum

Function

[CAPSP_BPT4] Capsid protein that self-associates to form 11 pentons, building the T=13 laevo capsid in association with 160 hexamers of gp23* and one dodecamer of gp20.^[1] ^[2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Gene product (gp) 24 of bacteriophage T4 forms the pentameric vertices of the capsid. Using x-ray crystallography, we found the principal domain of gp24 to have a polypeptide fold similar to that of the HK97 phage capsid protein plus an additional insertion domain. Fitting gp24 monomers into a cryo-EM density map of the mature T4 capsid suggests that the insertion domain interacts with a neighboring subunit, effecting a stabilization analogous to the covalent crosslinking in the HK97 capsid. Sequence alignment and genetic data show that the folds of gp24 and the hexamer-forming capsid protein, gp23*, are similar. Accordingly, models of gp24* pentamers, gp23* hexamers, and the whole capsid were built, based on a cryo-EM image reconstruction of the capsid. Mutations in gene 23 that affect capsid shape map to the capsomer's periphery, whereas mutations that allow gp23 to substitute for gp24 at the vertices modify the interactions between monomers within capsomers. Structural data show that capsid proteins of most tailed phages, and some eukaryotic viruses, may have evolved from a common ancestor.

Structural and functional similarities between the capsid proteins of bacteriophages T4 and HK97 point to a common ancestry.,Fokine A, Leiman PG, Shneider MM, Ahvazi B, Boeshans KM, Steven AC, Black LW, Mesyanzhinov VV, Rossmann MG Proc Natl Acad Sci U S A. 2005 May 17;102(20):7163-8. Epub 2005 May 6. PMID:15878991^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Olson NH, Gingery M, Eiserling FA, Baker TS. The structure of isometric capsids of bacteriophage T4. Virology. 2001 Jan 20;279(2):385-91. PMID:11162794 doi:http://dx.doi.org/10.1006/viro.2000.0735
↑ Fokine A, Chipman PR, Leiman PG, Mesyanzhinov VV, Rao VB, Rossmann MG. Molecular architecture of the prolate head of bacteriophage T4. Proc Natl Acad Sci U S A. 2004 Apr 20;101(16):6003-8. Epub 2004 Apr 7. PMID:15071181 doi:http://dx.doi.org/10.1073/pnas.0400444101
↑ Fokine A, Leiman PG, Shneider MM, Ahvazi B, Boeshans KM, Steven AC, Black LW, Mesyanzhinov VV, Rossmann MG. Structural and functional similarities between the capsid proteins of bacteriophages T4 and HK97 point to a common ancestry. Proc Natl Acad Sci U S A. 2005 May 17;102(20):7163-8. Epub 2005 May 6. PMID:15878991

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1yue"

@@ Line 2: / Line 2: @@
 <StructureSection load='1yue' size='340' side='right' caption='[[1yue]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1yue]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YUE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YUE FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1yue]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt4 Bpt4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YUE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YUE FirstGlance]. <br>
 </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">24 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 Enterobacteria phage T4])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">24 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 BPT4])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yue FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yue OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1yue RCSB], [http://www.ebi.ac.uk/pdbsum/1yue PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yue FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yue OCA], [http://pdbe.org/1yue PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1yue RCSB], [http://www.ebi.ac.uk/pdbsum/1yue PDBsum]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/VG24_BPT4 VG24_BPT4]] Capsid protein that self-associates to form 11 pentons, building the T=13 capsid head in association with 160 hexamers of gp23* and one dodecamer of gp20.
+[[http://www.uniprot.org/uniprot/CAPSP_BPT4 CAPSP_BPT4]] Capsid protein that self-associates to form 11 pentons, building the T=13 laevo capsid in association with 160 hexamers of gp23* and one dodecamer of gp20.<ref>PMID:11162794</ref> <ref>PMID:15071181</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 27: / Line 27: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 1yue" style="background-color:#fffaf0;"></div>
 ==See Also==
@@ Line 34: / Line 35: @@
 __TOC__
 </StructureSection>
-[[Category: Enterobacteria phage t4]]
+[[Category: Bpt4]]
 [[Category: Ahvazi, B]]
 [[Category: Black, L W]]

1yue

From Proteopedia

Revision as of 09:36, 10 September 2015

Bacteriophage T4 capsid vertex protein gp24

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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