1khw

Structural highlights

Function

[POLG_RHDVF] NTPase presumably plays a role in replication (By similarity).^{[1] [2]} Viral genome-linked protein is covalently linked to the 5'-end of the positive-strand, negative-strand genomic RNAs and subgenomic RNA. Acts as a genome-linked replication primer. May recruit ribosome to viral RNA thereby promoting viral proteins translation (By similarity).^{[3] [4]} 3C-like protease processes the polyprotein: 3CLpro-RdRp (p72) is first released by autocleavage, then all other proteins are cleaved (By similarity).^{[5] [6]} RNA-directed RNA polymerase replicates genomic and antigenomic RNA by recognizing replications specific signals. Transcribes also a subgenomic mRNA by initiating RNA synthesis internally on antigenomic RNA. This sgRNA codes for structural proteins. Catalyzes the covalent attachment VPg with viral RNAs (By similarity).^{[7] [8]} Capsid protein VP60 self assembles to form an icosahedral capsid with a T=3 symmetry, about 35 nm in diameter, and consisting of 180 capsid proteins. A smaller form of capsid with a diameter of 23 nm might be capsid proteins assembled as icosahedron with T=1 symmetry. The capsid encapsulate VP2 proteins and genomic or subgenomic RNA. Attaches virion to target cells by binding histo-blood group antigens, inducing endocytosis of the viral particle. Acidification of the endosome induces conformational change of capsid protein thereby injecting virus genomic RNA into host cytoplasm (By similarity).^{[9] [10]}

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

• RNA polymerase

References

1. ↑ Meyers G, Wirblich C, Thiel HJ. Genomic and subgenomic RNAs of rabbit hemorrhagic disease virus are both protein-linked and packaged into particles. Virology. 1991 Oct;184(2):677-86. PMID:1887589
2. ↑ Barcena J, Verdaguer N, Roca R, Morales M, Angulo I, Risco C, Carrascosa JL, Torres JM, Caston JR. The coat protein of Rabbit hemorrhagic disease virus contains a molecular switch at the N-terminal region facing the inner surface of the capsid. Virology. 2004 Apr 25;322(1):118-34. PMID:15063122 doi:http://dx.doi.org/10.1016/j.virol.2004.01.021
3. ↑ Meyers G, Wirblich C, Thiel HJ. Genomic and subgenomic RNAs of rabbit hemorrhagic disease virus are both protein-linked and packaged into particles. Virology. 1991 Oct;184(2):677-86. PMID:1887589
4. ↑ Barcena J, Verdaguer N, Roca R, Morales M, Angulo I, Risco C, Carrascosa JL, Torres JM, Caston JR. The coat protein of Rabbit hemorrhagic disease virus contains a molecular switch at the N-terminal region facing the inner surface of the capsid. Virology. 2004 Apr 25;322(1):118-34. PMID:15063122 doi:http://dx.doi.org/10.1016/j.virol.2004.01.021
5. ↑ Meyers G, Wirblich C, Thiel HJ. Genomic and subgenomic RNAs of rabbit hemorrhagic disease virus are both protein-linked and packaged into particles. Virology. 1991 Oct;184(2):677-86. PMID:1887589
6. ↑ Barcena J, Verdaguer N, Roca R, Morales M, Angulo I, Risco C, Carrascosa JL, Torres JM, Caston JR. The coat protein of Rabbit hemorrhagic disease virus contains a molecular switch at the N-terminal region facing the inner surface of the capsid. Virology. 2004 Apr 25;322(1):118-34. PMID:15063122 doi:http://dx.doi.org/10.1016/j.virol.2004.01.021
7. ↑ Meyers G, Wirblich C, Thiel HJ. Genomic and subgenomic RNAs of rabbit hemorrhagic disease virus are both protein-linked and packaged into particles. Virology. 1991 Oct;184(2):677-86. PMID:1887589
8. ↑ Barcena J, Verdaguer N, Roca R, Morales M, Angulo I, Risco C, Carrascosa JL, Torres JM, Caston JR. The coat protein of Rabbit hemorrhagic disease virus contains a molecular switch at the N-terminal region facing the inner surface of the capsid. Virology. 2004 Apr 25;322(1):118-34. PMID:15063122 doi:http://dx.doi.org/10.1016/j.virol.2004.01.021
9. ↑ Meyers G, Wirblich C, Thiel HJ. Genomic and subgenomic RNAs of rabbit hemorrhagic disease virus are both protein-linked and packaged into particles. Virology. 1991 Oct;184(2):677-86. PMID:1887589
10. ↑ Barcena J, Verdaguer N, Roca R, Morales M, Angulo I, Risco C, Carrascosa JL, Torres JM, Caston JR. The coat protein of Rabbit hemorrhagic disease virus contains a molecular switch at the N-terminal region facing the inner surface of the capsid. Virology. 2004 Apr 25;322(1):118-34. PMID:15063122 doi:http://dx.doi.org/10.1016/j.virol.2004.01.021
11. ↑ Ng KK, Cherney MM, Vazquez AL, Machin A, Alonso JM, Parra F, James MN. Crystal structures of active and inactive conformations of a caliciviral RNA-dependent RNA polymerase. J Biol Chem. 2002 Jan 11;277(2):1381-7. Epub 2001 Oct 24. PMID:11677245 doi:10.1074/jbc.M109261200