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1xeq

From Proteopedia

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Revision as of 23:04, 10 September 2015

Crystal tructure of RNA binding domain of influenza B virus non-structural protein

Structural highlights

1xeq is a 2 chain structure with sequence from Inble. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	1ns1
Gene:	8 (INBLE)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, TOPSAN

Function

[NS1_INBLE] Binds and inhibits the ubiquitin-like protein G1P2/ISG15, which is an early antiviral protein. Inhibits IRF-3 nuclear translocation and activation. Inhibits IFN-beta promoter activation; this inhibition is not dsRNA-binding dependent Prevents EIF2AK2/PKR activation, either by binding double strand RNA or by interacting directly with EIF2AK2/PKR. Also binds poly(A) and U6 snRNA. Suppresses the RNA silencing-based antiviral response in Drosophila cells.^[1]

Publication Abstract from PubMed

Influenza A viruses cause a highly contagious respiratory disease in humans and are responsible for periodic widespread epidemics with high mortality rates. The influenza A virus NS1 protein (NS1A) plays a key role in countering host antiviral defense and in virulence. The 73-residue N-terminal domain of NS1A (NS1A-(1-73)) forms a symmetric homodimer with a unique six-helical chain fold. It binds canonical A-form double-stranded RNA (dsRNA). Mutational inactivation of this dsRNA binding activity of NS1A highly attenuates virus replication. Here, we have characterized the unique structural features of the dsRNA binding surface of NS1A-(1-73) using NMR methods and describe the 2.1-A x-ray crystal structure of the corresponding dsRNA binding domain from human influenza B virus NS1B-(15-93). These results identify conserved dsRNA binding surfaces on both NS1A-(1-73) and NS1B-(15-93) that are very different from those indicated in earlier "working models" of the complex between dsRNA and NS1A-(1-73). The combined NMR and crystallographic data reveal highly conserved surface tracks of basic and hydrophilic residues that interact with dsRNA. These tracks are structurally complementary to the polyphosphate backbone conformation of A-form dsRNA and run at an approximately 45 degrees angle relative to the axes of helices alpha2/alpha2'. At the center of this dsRNA binding epitope, and common to NS1 proteins from influenza A and B viruses, is a deep pocket that includes both hydrophilic and hydrophobic amino acids. This pocket provides a target on the surface of the NS1 protein that is potentially suitable for the development of antiviral drugs targeting both influenza A and B viruses.

Conserved surface features form the double-stranded RNA binding site of non-structural protein 1 (NS1) from influenza A and B viruses.,Yin C, Khan JA, Swapna GV, Ertekin A, Krug RM, Tong L, Montelione GT J Biol Chem. 2007 Jul 13;282(28):20584-92. Epub 2007 May 1. PMID:17475623^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Donelan NR, Dauber B, Wang X, Basler CF, Wolff T, Garcia-Sastre A. The N- and C-terminal domains of the NS1 protein of influenza B virus can independently inhibit IRF-3 and beta interferon promoter activation. J Virol. 2004 Nov;78(21):11574-82. PMID:15479798 doi:78/21/11574
↑ Yin C, Khan JA, Swapna GV, Ertekin A, Krug RM, Tong L, Montelione GT. Conserved surface features form the double-stranded RNA binding site of non-structural protein 1 (NS1) from influenza A and B viruses. J Biol Chem. 2007 Jul 13;282(28):20584-92. Epub 2007 May 1. PMID:17475623 doi:10.1074/jbc.M611619200

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1xeq"

@@ Line 2: / Line 2: @@
 <StructureSection load='1xeq' size='340' side='right' caption='[[1xeq]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1xeq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Influenza_b_virus_(b/lee/40) Influenza b virus (b/lee/40)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XEQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XEQ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1xeq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Inble Inble]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XEQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XEQ FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ns1|1ns1]]</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">8 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=107412 Influenza B virus (B/Lee/40)])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">8 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=107412 INBLE])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xeq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xeq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1xeq RCSB], [http://www.ebi.ac.uk/pdbsum/1xeq PDBsum], [http://www.topsan.org/Proteins/NESGC/1xeq TOPSAN]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xeq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xeq OCA], [http://pdbe.org/1xeq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xeq RCSB], [http://www.ebi.ac.uk/pdbsum/1xeq PDBsum], [http://www.topsan.org/Proteins/NESGC/1xeq TOPSAN]</span></td></tr>
 </table>
+== Function ==
+[[http://www.uniprot.org/uniprot/NS1_INBLE NS1_INBLE]] Binds and inhibits the ubiquitin-like protein G1P2/ISG15, which is an early antiviral protein. Inhibits IRF-3 nuclear translocation and activation. Inhibits IFN-beta promoter activation; this inhibition is not dsRNA-binding dependent Prevents EIF2AK2/PKR activation, either by binding double strand RNA or by interacting directly with EIF2AK2/PKR. Also binds poly(A) and U6 snRNA. Suppresses the RNA silencing-based antiviral response in Drosophila cells.<ref>PMID:15479798</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 16: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 1xeq" style="background-color:#fffaf0;"></div>
 ==See Also==
@@ Line 23: / Line 26: @@
 __TOC__
 </StructureSection>
+[[Category: Inble]]
 [[Category: Khan, J A]]
 [[Category: Krug, R M]]

1xeq

From Proteopedia

Revision as of 23:04, 10 September 2015

Crystal tructure of RNA binding domain of influenza B virus non-structural protein

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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