This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

1c7v

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 05:20, 11 September 2015

NMR SOLUTION STRUCTURE OF THE CALCIUM-BOUND C-TERMINAL DOMAIN (W81-S161) OF CALCIUM VECTOR PROTEIN FROM AMPHIOXUS

Structural highlights

1c7v is a 1 chain structure with sequence from Amphioxus lanceolatum. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1c7w
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum

Function

[CAVP_BRALA] The exact function of this protein is not yet known. It interacts with CAVPT, a protein also of unknown function, in a calcium-dependent way. This protein binds two calcium ions.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Calcium vector protein (CaVP) from amphioxus is a two-domain, calcium-binding protein (18.3 kDa) of the calmodulin superfamily. Only two of the four EF-hand motifs (sites III and IV) have a significant binding affinity for calcium ions. We determined the solution structure of the domain containing these active sites (C-CaVP: W81-S161), in the Ca(2+)-saturated state, using NMR spectroscopy and restrained molecular dynamics. The tertiary structure is similar to other Ca(2+)-binding domains containing a pair of EF-hand motifs. The apo state has spectroscopic and thermodynamic characteristics of a molten globule, with conserved secondary structure but highly fluctuating tertiary organization. Titration of C-CaVP with Ca(2+) revealed a stepwise ion binding, with a stable equilibrium intermediate in which only site III binds a calcium ion. Despite a highly fluctuating structure of the free site IV, the calcium-bound site III has a persistent structure, with similar secondary elements but different interhelix angle and hydrophobic packing relative to the fully calcium-saturated state.

Sequential calcium binding to the regulatory domain of calcium vector protein reveals functional asymmetry and a novel mode of structural rearrangement.,Theret I, Baladi S, Cox JA, Sakamoto H, Craescu CT Biochemistry. 2000 Jul 11;39(27):7920-6. PMID:10891072^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Theret I, Baladi S, Cox JA, Sakamoto H, Craescu CT. Sequential calcium binding to the regulatory domain of calcium vector protein reveals functional asymmetry and a novel mode of structural rearrangement. Biochemistry. 2000 Jul 11;39(27):7920-6. PMID:10891072

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1c7v"

@@ Line 2: / Line 2: @@
 <StructureSection load='1c7v' size='340' side='right' caption='[[1c7v]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1c7v]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Branchiostoma_lanceolatum Branchiostoma lanceolatum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C7V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1C7V FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1c7v]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Amphioxus_lanceolatum Amphioxus lanceolatum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C7V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1C7V FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1c7w|1c7w]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1c7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c7v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1c7v RCSB], [http://www.ebi.ac.uk/pdbsum/1c7v PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1c7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c7v OCA], [http://pdbe.org/1c7v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1c7v RCSB], [http://www.ebi.ac.uk/pdbsum/1c7v PDBsum]</span></td></tr>
 </table>
 == Function ==
@@ Line 26: / Line 26: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 1c7v" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Branchiostoma lanceolatum]]
+[[Category: Amphioxus lanceolatum]]
 [[Category: Baladi, S]]
 [[Category: Cox, J A]]

1c7v

From Proteopedia

Revision as of 05:20, 11 September 2015

NMR SOLUTION STRUCTURE OF THE CALCIUM-BOUND C-TERMINAL DOMAIN (W81-S161) OF CALCIUM VECTOR PROTEIN FROM AMPHIOXUS

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox