1vf6

From Proteopedia

(Difference between revisions)

Revision as of 10:17, 11 September 2015

2.1 Angstrom crystal structure of the PALS-1-L27N and PATJ L27 heterodimer complex

Structural highlights

1vf6 is a 4 chain structure with sequence from Human and Lk3 transgenic mice. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1rso
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum

Function

[INADL_HUMAN] Scaffolding protein that may bring different proteins into adjacent positions at the cell membrane. May regulate protein targeting, cell polarity and integrity of tight junctions. May regulate the surface expression and/or function of ASIC3 in sensory neurons.^[1] [MPP5_MOUSE] May play a role in tight junctions biogenesis and in the establishment of cell polarity in epithelial cells. May modulate SC6A1/GAT1-mediated GABA uptake by stabilizing the transporter. Required for localization of EZR to the apical membrane of parietal cells and may play a role in the dynamic remodeling of the apical cytoskeleton.^[2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

L27 is a protein-binding domain that can assemble essential proteins for signaling and cell polarity into complexes by interacting in a heterodimeric manner. One of these protein complexes is the PATJ/PALS1/Crumbs tripartite complex, which is crucial for the establishment and maintenance of cell polarity. To reveal the structural basis underlining the obligate heterodimerization, we have determined the crystal structure of the PALS1-L27N/PATJ-L27 heterodimer complex. Each L27 domain is composed of three helices. The two L27 domains heterodimerize by building a compact structure consisting of a four-helix bundle formed by the first two helices of each L27 domain and one coiled-coil formed by the third helix of each domain. The large hydrophobic packing interactions contributed by all the helices of both L27 domains predominantly drive the heterodimer formation, which is likely to be a general feature of L27 domains. Combined with mutational studies, we can begin to understand the structural basis for the specificity of L27 binding pairs. Our results provide unique insights into L27 domain heterodimer complex, which is critical for cell polarization.

Structural basis for L27 domain-mediated assembly of signaling and cell polarity complexes.,Li Y, Karnak D, Demeler B, Margolis B, Lavie A EMBO J. 2004 Jul 21;23(14):2723-33. Epub 2004 Jul 8. PMID:15241471^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Roh MH, Makarova O, Liu CJ, Shin K, Lee S, Laurinec S, Goyal M, Wiggins R, Margolis B. The Maguk protein, Pals1, functions as an adapter, linking mammalian homologues of Crumbs and Discs Lost. J Cell Biol. 2002 Apr 1;157(1):161-72. Epub 2002 Apr 1. PMID:11927608 doi:http://dx.doi.org/10.1083/jcb.200109010
↑ Cao X, Ding X, Guo Z, Zhou R, Wang F, Long F, Wu F, Bi F, Wang Q, Fan D, Forte JG, Teng M, Yao X. PALS1 specifies the localization of ezrin to the apical membrane of gastric parietal cells. J Biol Chem. 2005 Apr 8;280(14):13584-92. Epub 2005 Jan 27. PMID:15677456 doi:http://dx.doi.org/10.1074/jbc.M411941200
↑ Li Y, Karnak D, Demeler B, Margolis B, Lavie A. Structural basis for L27 domain-mediated assembly of signaling and cell polarity complexes. EMBO J. 2004 Jul 21;23(14):2723-33. Epub 2004 Jul 8. PMID:15241471 doi:10.1038/sj.emboj.7600294

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1vf6"

@@ Line 2: / Line 2: @@
 <StructureSection load='1vf6' size='340' side='right' caption='[[1vf6]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1vf6]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VF6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1VF6 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1vf6]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VF6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1VF6 FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1rso|1rso]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vf6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vf6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1vf6 RCSB], [http://www.ebi.ac.uk/pdbsum/1vf6 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vf6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vf6 OCA], [http://pdbe.org/1vf6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1vf6 RCSB], [http://www.ebi.ac.uk/pdbsum/1vf6 PDBsum]</span></td></tr>
 </table>
 == Function ==
@@ Line 26: / Line 26: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 1vf6" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Homo sapiens]]
+[[Category: Human]]
-[[Category: Mus musculus]]
+[[Category: Lk3 transgenic mice]]
 [[Category: Karnak, D]]
 [[Category: Lavie, A]]

1vf6

From Proteopedia

Revision as of 10:17, 11 September 2015

2.1 Angstrom crystal structure of the PALS-1-L27N and PATJ L27 heterodimer complex

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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