1od7
From Proteopedia
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==Overview== | ==Overview== | ||
| - | The Siglec family of receptors mediates cell surface interactions through, recognition of sialylated glycoconjugates. The crystal structure of the, N-terminal immunoglobulin-like domain of the Siglec sialoadhesin (SnD1) in, complex with 2,3-sialyllactose has informed the design of sialic acid, analogs (sialosides) that bind Siglecs with significantly enhanced, affinities and specificities. Binding assays against sialoadhesin (Sn;, Siglec-1), CD22 (Siglec-2), and MAG (Siglec-4) show a 10- to 300-fold, reduction in IC(50) values (relative to methyl-alpha-Neu5Ac) for three, sialosides bearing aromatic group modifications of the glycerol side, chain: Me-alpha-9-N-benzoyl-amino-9-deoxy-Neu5Ac (BENZ), Me-alpha-9-N-(naphthyl-2-carbonyl)-amino-9-deoxy-Neu5Ac (NAP), and, .. | + | The Siglec family of receptors mediates cell surface interactions through, recognition of sialylated glycoconjugates. The crystal structure of the, N-terminal immunoglobulin-like domain of the Siglec sialoadhesin (SnD1) in, complex with 2,3-sialyllactose has informed the design of sialic acid, analogs (sialosides) that bind Siglecs with significantly enhanced, affinities and specificities. Binding assays against sialoadhesin (Sn;, Siglec-1), CD22 (Siglec-2), and MAG (Siglec-4) show a 10- to 300-fold, reduction in IC(50) values (relative to methyl-alpha-Neu5Ac) for three, sialosides bearing aromatic group modifications of the glycerol side, chain: Me-alpha-9-N-benzoyl-amino-9-deoxy-Neu5Ac (BENZ), Me-alpha-9-N-(naphthyl-2-carbonyl)-amino-9-deoxy-Neu5Ac (NAP), and, Me-alpha-9-N-(biphenyl-4-carbonyl)-amino-9-deoxy-Neu5Ac (BIP). Crystal, structures of these sialosides in complex with SnD1 suggest explanations, for the differences in specificity and affinity, providing further ideas, for compound design of physiological and potentially therapeutic, relevance. |
==About this Structure== | ==About this Structure== | ||
| - | 1OD7 is a | + | 1OD7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with SUW as [http://en.wikipedia.org/wiki/ligand ligand]. Structure known Active Site: SUW. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OD7 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: siglec]] | [[Category: siglec]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 14:50:27 2007'' |
Revision as of 12:45, 5 November 2007
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N-TERMINAL OF SIALOADHESIN IN COMPLEX WITH ME-A-9-N-(NAPHTHYL-2-CARBONYL)-AMINO-9-DEOXY-NEU5AC (NAP COMPOUND)
Overview
The Siglec family of receptors mediates cell surface interactions through, recognition of sialylated glycoconjugates. The crystal structure of the, N-terminal immunoglobulin-like domain of the Siglec sialoadhesin (SnD1) in, complex with 2,3-sialyllactose has informed the design of sialic acid, analogs (sialosides) that bind Siglecs with significantly enhanced, affinities and specificities. Binding assays against sialoadhesin (Sn;, Siglec-1), CD22 (Siglec-2), and MAG (Siglec-4) show a 10- to 300-fold, reduction in IC(50) values (relative to methyl-alpha-Neu5Ac) for three, sialosides bearing aromatic group modifications of the glycerol side, chain: Me-alpha-9-N-benzoyl-amino-9-deoxy-Neu5Ac (BENZ), Me-alpha-9-N-(naphthyl-2-carbonyl)-amino-9-deoxy-Neu5Ac (NAP), and, Me-alpha-9-N-(biphenyl-4-carbonyl)-amino-9-deoxy-Neu5Ac (BIP). Crystal, structures of these sialosides in complex with SnD1 suggest explanations, for the differences in specificity and affinity, providing further ideas, for compound design of physiological and potentially therapeutic, relevance.
About this Structure
1OD7 is a Single protein structure of sequence from Mus musculus with SUW as ligand. Structure known Active Site: SUW. Full crystallographic information is available from OCA.
Reference
Structure-guided design of sialic acid-based Siglec inhibitors and crystallographic analysis in complex with sialoadhesin., Zaccai NR, Maenaka K, Maenaka T, Crocker PR, Brossmer R, Kelm S, Jones EY, Structure. 2003 May;11(5):557-67. PMID:12737821
Page seeded by OCA on Mon Nov 5 14:50:27 2007
