1soi
From Proteopedia
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|PDB= 1soi |SIZE=350|CAPTION= <scene name='initialview01'>1soi</scene>, resolution 1.8Å | |PDB= 1soi |SIZE=350|CAPTION= <scene name='initialview01'>1soi</scene>, resolution 1.8Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=SM:SAMARIUM (III) ION'>SM</scene> | + | |LIGAND= <scene name='pdbligand=SM:SAMARIUM+(III)+ION'>SM</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= DR1025 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1299 Deinococcus radiodurans]) | |GENE= DR1025 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1299 Deinococcus radiodurans]) | ||
| + | |DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd04700 DR1025_like]</span> | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1soi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1soi OCA], [http://www.ebi.ac.uk/pdbsum/1soi PDBsum], [http://www.fli-leibniz.de/cgi-bin/ImgLib.pl?CODE=1kfv JenaLib], [http://www.rcsb.org/pdb/explore.do?structureId=1soi RCSB]</span> | ||
}} | }} | ||
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[[Category: Schulze-Gahmen, U.]] | [[Category: Schulze-Gahmen, U.]] | ||
[[Category: Xu, W.]] | [[Category: Xu, W.]] | ||
| - | [[Category: SM]] | ||
[[Category: alpha-beta-alpha sandwich]] | [[Category: alpha-beta-alpha sandwich]] | ||
[[Category: berkeley structural genomics center]] | [[Category: berkeley structural genomics center]] | ||
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[[Category: structural genomic]] | [[Category: structural genomic]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 26 06:05:32 2008'' |
Revision as of 04:05, 26 March 2008
| |||||||
| , resolution 1.8Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | DR1025 (Deinococcus radiodurans) | ||||||
| Domains: | DR1025_like | ||||||
| Resources: | FirstGlance, OCA, PDBsum, JenaLib, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF NUDIX HYDROLASE DR1025 IN COMPLEX WITH SM+3
Overview
We have determined the crystal structure, at 1.4A, of the Nudix hydrolase DR1025 from the extremely radiation resistant bacterium Deinococcus radiodurans. The protein forms an intertwined homodimer by exchanging N-terminal segments between chains. We have identified additional conserved elements of the Nudix fold, including the metal-binding motif, a kinked beta-strand characterized by a proline two positions upstream of the Nudix consensus sequence, and participation of the N-terminal extension in the formation of the substrate-binding pocket. Crystal structures were also solved of DR1025 crystallized in the presence of magnesium and either a GTP analog or Ap(4)A (both at 1.6A resolution). In the Ap(4)A co-crystal, the electron density indicated that the product of asymmetric hydrolysis, ATP, was bound to the enzyme. The GTP analog bound structure showed that GTP was bound almost identically as ATP. Neither nucleoside triphosphate was further cleaved.
About this Structure
1SOI is a Single protein structure of sequence from Deinococcus radiodurans. Full crystallographic information is available from OCA.
Reference
Structural studies of the Nudix hydrolase DR1025 from Deinococcus radiodurans and its ligand complexes., Ranatunga W, Hill EE, Mooster JL, Holbrook EL, Schulze-Gahmen U, Xu W, Bessman MJ, Brenner SE, Holbrook SR, J Mol Biol. 2004 May 21;339(1):103-16. PMID:15123424
Page seeded by OCA on Wed Mar 26 06:05:32 2008
Categories: Deinococcus radiodurans | Single protein | BSGC, Berkeley Structural Genomics Center. | Bessman, M J. | Brenner, S E. | Hill, E E. | Holbrook, E L. | Holbrook, S R. | Mooster, J L. | Ranatunga, W. | Schulze-Gahmen, U. | Xu, W. | Alpha-beta-alpha sandwich | Berkeley structural genomics center | Bsgc structure funded by nih | Nudix fold | Protein structure initiative | Psi | Structural genomic
