This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

4azl

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 06:57, 30 September 2015

In meso structure of alginate transporter, AlgE, from Pseudomoas aeruginosa, PAO1, crystal form 2.

Structural highlights

4azl is a 2 chain structure with sequence from Pseae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Related:	4afk
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum

Function

[ALGE_PSEAE] Has non-porin-like, channel-forming properties and probably functions as an alginate permeability pore.

Publication Abstract from PubMed

The exopolysaccharide alginate is an important component of biofilms produced by Pseudomonas aeruginosa, a major pathogen that contributes to the demise of cystic fibrosis patients. Alginate exits the cell via the outer membrane porin AlgE. X-ray structures of several AlgE crystal forms are reported here. Whilst all share a common beta-barrel constitution, they differ in the degree to which loops L2 and T8 are ordered. L2 and T8 have been identified as an extracellular gate (E-gate) and a periplasmic gate (P-gate), respectively, that reside on either side of an alginate-selectivity pore located midway through AlgE. Passage of alginate across the membrane is proposed to be regulated by the sequential opening and closing of the two gates. In one crystal form, the selectivity pore contains a bound citrate. Because citrate mimics the uronate monomers of alginate, its location is taken to highlight a route through AlgE taken by alginate as it crosses the pore. Docking and molecular-dynamics simulations support and extend the proposed transport mechanism. Specifically, the P-gate and E-gate are flexible and move between open and closed states. Citrate can leave the selectivity pore bidirectionally. Alginate docks stably in a linear conformation through the open pore. To translate across the pore, a force is required that presumably is provided by the alginate-synthesis machinery. Accessing the open pore is facilitated by complex formation between AlgE and the periplasmic protein AlgK. Alginate can thread through a continuous pore in the complex, suggesting that AlgK pre-orients newly synthesized exopolysaccharide for delivery to AlgE.

A conformational landscape for alginate secretion across the outer membrane of Pseudomonas aeruginosa.,Tan J, Rouse SL, Li D, Pye VE, Vogeley L, Brinth AR, El Arnaout T, Whitney JC, Howell PL, Sansom MS, Caffrey M Acta Crystallogr D Biol Crystallogr. 2014 Aug 1;70(Pt 8):2054-68. doi:, 10.1107/S1399004714001850. Epub 2014 Jul 25. PMID:25084326^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Tan J, Rouse SL, Li D, Pye VE, Vogeley L, Brinth AR, El Arnaout T, Whitney JC, Howell PL, Sansom MS, Caffrey M. A conformational landscape for alginate secretion across the outer membrane of Pseudomonas aeruginosa. Acta Crystallogr D Biol Crystallogr. 2014 Aug 1;70(Pt 8):2054-68. doi:, 10.1107/S1399004714001850. Epub 2014 Jul 25. PMID:25084326 doi:http://dx.doi.org/10.1107/S1399004714001850

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4azl"

@@ Line 5: / Line 5: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=78M:(2S)-2,3-DIHYDROXYPROPYL(7Z)-PENTADEC-7-ENOATE'>78M</scene>, <scene name='pdbligand=78N:(2R)-2,3-DIHYDROXYPROPYL(7Z)-PENTADEC-7-ENOATE'>78N</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4afk|4afk]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4azl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4azl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4azl RCSB], [http://www.ebi.ac.uk/pdbsum/4azl PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4azl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4azl OCA], [http://pdbe.org/4azl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4azl RCSB], [http://www.ebi.ac.uk/pdbsum/4azl PDBsum]</span></td></tr>
 </table>
 == Function ==
@@ Line 17: / Line 17: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4azl" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

4azl

From Proteopedia

Revision as of 06:57, 30 September 2015

In meso structure of alginate transporter, AlgE, from Pseudomoas aeruginosa, PAO1, crystal form 2.

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox