2cj8
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Plant acid invertases catalyse the breakdown of sucrose. Their activity is, tightly regulated through interaction with specific protein inhibitors., The complex between the cell-wall invertase inhibitor Nt-CIF and its, target enzyme is stable only at acidic pH, as found in the plant cell, wall. Since the pH in this compartment can be modulated between pH 4 and 6, in planta, the rapid dissociation of the inhibitor-enzyme complex at, neutral pH may represent a regulatory event. Here, it is analyzed whether, the inhibitory component undergoes structural rearrangements upon changes, in the pH environment. Six crystal forms grown at pH 4.6-9.5 and, diffracting up to 1.63 Angstrom indicate only small structural changes in, CIF. This suggests that complex dissociation at neutral pH is mediated, . | + | Plant acid invertases catalyse the breakdown of sucrose. Their activity is, tightly regulated through interaction with specific protein inhibitors., The complex between the cell-wall invertase inhibitor Nt-CIF and its, target enzyme is stable only at acidic pH, as found in the plant cell, wall. Since the pH in this compartment can be modulated between pH 4 and 6, in planta, the rapid dissociation of the inhibitor-enzyme complex at, neutral pH may represent a regulatory event. Here, it is analyzed whether, the inhibitory component undergoes structural rearrangements upon changes, in the pH environment. Six crystal forms grown at pH 4.6-9.5 and, diffracting up to 1.63 Angstrom indicate only small structural changes in, CIF. This suggests that complex dissociation at neutral pH is mediated, either by rearrangements in the enzyme or by a complex pattern of surface, charges in the inhibitor-enzyme binding interface. |
==About this Structure== | ==About this Structure== | ||
| - | 2CJ8 is a | + | 2CJ8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum] with IOD as [http://en.wikipedia.org/wiki/ligand ligand]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CJ8 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: protein binding]] | [[Category: protein binding]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 14:56:32 2007'' |
Revision as of 12:51, 5 November 2007
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CRYSTAL STRUCTURE OF A CELL WALL INVERTASE INHIBITOR FROM TOBACCO (PH 9.5)
Overview
Plant acid invertases catalyse the breakdown of sucrose. Their activity is, tightly regulated through interaction with specific protein inhibitors., The complex between the cell-wall invertase inhibitor Nt-CIF and its, target enzyme is stable only at acidic pH, as found in the plant cell, wall. Since the pH in this compartment can be modulated between pH 4 and 6, in planta, the rapid dissociation of the inhibitor-enzyme complex at, neutral pH may represent a regulatory event. Here, it is analyzed whether, the inhibitory component undergoes structural rearrangements upon changes, in the pH environment. Six crystal forms grown at pH 4.6-9.5 and, diffracting up to 1.63 Angstrom indicate only small structural changes in, CIF. This suggests that complex dissociation at neutral pH is mediated, either by rearrangements in the enzyme or by a complex pattern of surface, charges in the inhibitor-enzyme binding interface.
About this Structure
2CJ8 is a Single protein structure of sequence from Nicotiana tabacum with IOD as ligand. Structure known Active Site: AC1. Full crystallographic information is available from OCA.
Reference
Multiple crystal forms of the cell-wall invertase inhibitor from tobacco support high conformational rigidity over a broad pH range., Hothorn M, Scheffzek K, Acta Crystallogr D Biol Crystallogr. 2006 Jun;62(Pt 6):665-70. Epub 2006, May 12. PMID:16699194
Page seeded by OCA on Mon Nov 5 14:56:32 2007
