This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
4zm4
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | ''' | + | ==Complex structure of PctV K276R mutant with PMP and 3-dehydroshkimate== |
| + | <StructureSection load='4zm4' size='340' side='right' caption='[[4zm4]], [[Resolution|resolution]] 2.40Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4zm4]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZM4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZM4 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=P3B:(3E,4R,5R)-4,5-DIHYDROXY-3-{[(Z)-{3-HYDROXY-2-METHYL-5-[(PHOSPHONOOXY)METHYL]PYRIDIN-4(1H)-YLIDENE}METHYL]IMINO}CYCLOHEX-1-ENE-1-CARBOXYLIC+ACID'>P3B</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4zm3|4zm3]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zm4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zm4 OCA], [http://pdbe.org/4zm4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zm4 RCSB], [http://www.ebi.ac.uk/pdbsum/4zm4 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Mutational analysis of the pyridoxal 5'-phosphate (PLP)-dependent enzyme PctV has been carried out to elucidate the multi-step reaction mechanism for the formation of 3-aminobenzoate (3-ABA) from 3-dehydroshikimate (3-DSA). The results revealed that the introduction of a K276R mutation led to the accumulation of a quinonoid intermediate with an absorption maximum of 580 nm following the reaction of pyridoxamine 5'-phosphate (PMP) with 3-DSA. Furthermore, the chemical structure of this intermediate was supported by X-ray crystallographic analysis of the complex formed between the K276R mutant and the quinonoid intermediate. These results clearly show that a quinonoid intermediate is involved in the formation of 3-ABA, and also indicate that the Lys276 residue in the active site of the PctV enzyme plays multiple roles including acid/base catalysis during the dehydration reaction of the quinonoid intermediate, as well as assisting in the PMP formation and aminotransfer reaction. | ||
| - | + | Mechanism-based Trapping of the Quinonoid Intermediate Using the K276R Mutant of PLP-Dependent 3-Aminobenzoate Synthase PctV in the Biosynthesis of Pactamycin.,Hirayama A, Miyanaga A, Kudo F, Eguchi T Chembiochem. 2015 Oct 1. doi: 10.1002/cbic.201500426. PMID:26426567<ref>PMID:26426567</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 4zm4" style="background-color:#fffaf0;"></div> | |
| - | [[Category: | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Eguchi, T]] | ||
| + | [[Category: Hirayama, A]] | ||
[[Category: Kudo, F]] | [[Category: Kudo, F]] | ||
| - | [[Category: Hirayama, A]] | ||
| - | [[Category: Eguchi, T]] | ||
[[Category: Miyanaga, A]] | [[Category: Miyanaga, A]] | ||
| + | [[Category: Aminotransferase]] | ||
| + | [[Category: Pyridoxal 5-phosphate]] | ||
| + | [[Category: Transferase]] | ||
Revision as of 03:53, 16 October 2015
Complex structure of PctV K276R mutant with PMP and 3-dehydroshkimate
| |||||||||||
