DNA polymerase
From Proteopedia
| Line 337: | Line 337: | ||
**[[2lsk]] - mDpo κ + Rev1<br /> | **[[2lsk]] - mDpo κ + Rev1<br /> | ||
| - | + | *Terminal deoxynucleotidyl transferase | |
**[[2coe]] – TdT BRCT domain – NMR<br /> | **[[2coe]] – TdT BRCT domain – NMR<br /> | ||
**[[1jms]] – mTdT catalytic domain + Mg <br / > | **[[1jms]] – mTdT catalytic domain + Mg <br / > | ||
| - | Terminal deoxynucleotidyl transferase binary complex | + | *Terminal deoxynucleotidyl transferase binary complex |
**[[4iqt]], [[4iqu]], [[4iqv]], [[4iqw]] – mTdT catalytic domain + inhibitor <br / > | **[[4iqt]], [[4iqu]], [[4iqv]], [[4iqw]] – mTdT catalytic domain + inhibitor <br / > | ||
| Line 354: | Line 354: | ||
**[[4i2a]] – mTdT catalytic domain + UMP derivative + Mg <br / > | **[[4i2a]] – mTdT catalytic domain + UMP derivative + Mg <br / > | ||
| - | Terminal deoxynucleotidyl transferase ternary complex with DNA | + | *Terminal deoxynucleotidyl transferase ternary complex with DNA |
**[[4i2e]] – mTdT catalytic domain + AMPCPP + Co + DNA <br / > | **[[4i2e]] – mTdT catalytic domain + AMPCPP + Co + DNA <br / > | ||
Revision as of 11:17, 20 October 2015
DNA polymerase (Dpo) reads a DNA strand and catalyzes the synthesis of its complementary strand. Dpo's are divided into 7 families according to their sequence homology and 3D structure similarities. The families are:
- Family A - replicative or repair Dpo.
- Family B - replicative Dpo involved in processing DNA replication during cell division (includes eukaryotic Dpo α,δ,ε).
- Family C - major replicative Dpo in bacteria (includes Dpo II, III, IV).
- Family D - replicative. Present in archaea.
- Family X - eukaryotic repair Dpo (includes Dpo β,λ,σ,μ and terminal deoxynucleotidyl transferase).
- Family Y - replicative of damaged DNA (includes eukaryotic η,ι,κ).
- Family RT - reverse transcriptase. See Reverse transcriptase.
Some Dpo terminology:
Dpo sliding clamp is made of the complex of Dpo and Proliferating Cell Nuclear Antigen (PCNA) which encircles it.
The BRCT domain in Dpo is the C-terminal domain of breast cancer susceptibility protein.
Klenow fragment is a large Dpo fragment produced upon cleavage of Dpo by subtilisin.
In the E. coli, the EcDpo III subunits β, γ, δ, δ' are named clamp loader. This complex assembles the β subunit sliding clamp unto the DNA.
Rev1 is a Dpo involved in replication over DNA lesions.
Terminal deoxynucleotidyl transferase (TdT) is a family X Dpo expressed in immature lymphoid cells. TdT adds nucleotides to exons during antibody gene recombination.
For more details see
- DNA Polymerase I
- Polymerase III homoenzyme beta subunit
- User:Karl E. Zahn/RB69 DNA polymerase (gp43)
- Alpha Subunit of Thermus aquaticus DNA Polymerase III.
3D Structures of DNA polymerase
Updated on 20-October-2015
Proteopedia Page Contributors and Editors (what is this?)
Michal Harel, Cori Damron, Alexander Berchansky, Jaime Prilusky, Emily Bray, Joel L. Sussman, David Canner
