2h0n
From Proteopedia
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{{Structure | {{Structure | ||
|PDB= 2h0n |SIZE=350|CAPTION= <scene name='initialview01'>2h0n</scene>, resolution 1.53Å | |PDB= 2h0n |SIZE=350|CAPTION= <scene name='initialview01'>2h0n</scene>, resolution 1.53Å | ||
| - | |SITE= | + | |SITE= <scene name='pdbsite=AC1:2hp+Binding+Site+For+Residue+A+9'>AC1</scene> and <scene name='pdbsite=AC2:2hp+Binding+Site+For+Residue+B+9'>AC2</scene> |
| - | |LIGAND= <scene name='pdbligand=2HP:DIHYDROGENPHOSPHATE ION'>2HP</scene> | + | |LIGAND= <scene name='pdbligand=2HP:DIHYDROGENPHOSPHATE+ION'>2HP</scene>, <scene name='pdbligand=XAL:[(1S,4R,6R)-6-HYDROXY-4-(ADENIN-9-YL)CYCLOHEX-2-EN-1-YL]METHYL+DIHYDROGEN+PHOSPHATE'>XAL</scene>, <scene name='pdbligand=XCL:[(1S,4R,6R)-6-HYDROXY-4-(CYTOSIN-9-YL)CYCLOHEX-2-EN-1-YL]METHYL+DIHYDROGEN+PHOSPHATE'>XCL</scene>, <scene name='pdbligand=XGL:[(1S,4R,6R)-6-HYDROXY-4-(GUANIN-9-YL)CYCLOHEX-2-EN-1-YL]METHYL+DIHYDROGEN+PHOSPHATE'>XGL</scene>, <scene name='pdbligand=XTL:[(1S,4R,6R)-6-HYDROXY-4-(THYMIN-9-YL)CYCLOHEX-2-EN-1-YL]METHYL+DIHYDROGEN+PHOSPHATE'>XTL</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h0n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h0n OCA], [http://www.ebi.ac.uk/pdbsum/2h0n PDBsum], [http://www.fli-leibniz.de/cgi-bin/ImgLib.pl?CODE=1kfv JenaLib], [http://www.rcsb.org/pdb/explore.do?structureId=2h0n RCSB]</span> | ||
}} | }} | ||
'''Structure of the fully modified left-handed cyclohexene nucleic acid sequence GTGTACAC''' | '''Structure of the fully modified left-handed cyclohexene nucleic acid sequence GTGTACAC''' | ||
| + | |||
| + | ==Overview== | ||
| + | CeNA oligonucleotides consist of a phosphorylated backbone where the deoxyribose sugars are replaced by cyclohexene moieties. The X-ray structure determination and analysis of a fully modified octamer sequence GTGTACAC, which is the first crystal structure of a carbocyclic-based nucleic acid, is presented. This particular sequence was built with left-handed building blocks and crystallizes as a left-handed double helix. The helix can be characterized as belonging to the (mirrored) A-type family. Crystallographic data were processed up to 1.53 A, and the octamer sequence crystallizes in the space group R32. The sugar puckering is found to adopt the 3H2 half-chair conformation which mimics the C3'-endo conformation of the ribose sugar. The double helices stack on top of each other to form continuous helices, and static disorder is observed due to this end-to-end stacking. | ||
==About this Structure== | ==About this Structure== | ||
2H0N is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H0N OCA]. | 2H0N is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H0N OCA]. | ||
| + | |||
| + | ==Reference== | ||
| + | Structure of the fully modified left-handed cyclohexene nucleic acid sequence GTGTACAC., Robeyns K, Herdewijn P, Van Meervelt L, J Am Chem Soc. 2008 Feb 13;130(6):1979-84. Epub 2008 Jan 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18198873 18198873] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Meervelt, L Van.]] | [[Category: Meervelt, L Van.]] | ||
[[Category: Robeyns, K.]] | [[Category: Robeyns, K.]] | ||
| - | [[Category: 2HP]] | ||
[[Category: cyclohexene nucleic acid]] | [[Category: cyclohexene nucleic acid]] | ||
[[Category: dna analogue]] | [[Category: dna analogue]] | ||
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[[Category: left-handed]] | [[Category: left-handed]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 26 10:01:31 2008'' |
Revision as of 08:01, 26 March 2008
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| , resolution 1.53Å | |||||||
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| Sites: | and | ||||||
| Ligands: | , , , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, JenaLib, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of the fully modified left-handed cyclohexene nucleic acid sequence GTGTACAC
Overview
CeNA oligonucleotides consist of a phosphorylated backbone where the deoxyribose sugars are replaced by cyclohexene moieties. The X-ray structure determination and analysis of a fully modified octamer sequence GTGTACAC, which is the first crystal structure of a carbocyclic-based nucleic acid, is presented. This particular sequence was built with left-handed building blocks and crystallizes as a left-handed double helix. The helix can be characterized as belonging to the (mirrored) A-type family. Crystallographic data were processed up to 1.53 A, and the octamer sequence crystallizes in the space group R32. The sugar puckering is found to adopt the 3H2 half-chair conformation which mimics the C3'-endo conformation of the ribose sugar. The double helices stack on top of each other to form continuous helices, and static disorder is observed due to this end-to-end stacking.
About this Structure
2H0N is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Structure of the fully modified left-handed cyclohexene nucleic acid sequence GTGTACAC., Robeyns K, Herdewijn P, Van Meervelt L, J Am Chem Soc. 2008 Feb 13;130(6):1979-84. Epub 2008 Jan 17. PMID:18198873
Page seeded by OCA on Wed Mar 26 10:01:31 2008
