1a8s
From Proteopedia
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|PDB= 1a8s |SIZE=350|CAPTION= <scene name='initialview01'>1a8s</scene>, resolution 1.80Å | |PDB= 1a8s |SIZE=350|CAPTION= <scene name='initialview01'>1a8s</scene>, resolution 1.80Å | ||
|SITE= <scene name='pdbsite=NUL:Catalytic+Triad'>NUL</scene> | |SITE= <scene name='pdbsite=NUL:Catalytic+Triad'>NUL</scene> | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=PPI:PROPANOIC+ACID'>PPI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Chloride_peroxidase Chloride peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.10 1.11.1.10] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Chloride_peroxidase Chloride peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.10 1.11.1.10] </span> |
|GENE= CPOF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=294 Pseudomonas fluorescens]) | |GENE= CPOF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=294 Pseudomonas fluorescens]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a8s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a8s OCA], [http://www.ebi.ac.uk/pdbsum/1a8s PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1a8s RCSB]</span> | ||
}} | }} | ||
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[[Category: Pelletier, I.]] | [[Category: Pelletier, I.]] | ||
[[Category: Toelzer, S.]] | [[Category: Toelzer, S.]] | ||
| - | [[Category: PPI]] | ||
| - | [[Category: SO4]] | ||
[[Category: haloperoxidase]] | [[Category: haloperoxidase]] | ||
[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
[[Category: propionate complex]] | [[Category: propionate complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:35:43 2008'' |
Revision as of 15:35, 30 March 2008
| |||||||
| , resolution 1.80Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | |||||||
| Ligands: | , | ||||||
| Gene: | CPOF (Pseudomonas fluorescens) | ||||||
| Activity: | Chloride peroxidase, with EC number 1.11.1.10 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CHLOROPEROXIDASE F/PROPIONATE COMPLEX
Overview
The structures of cofactor-free haloperoxidases from Streptomyces aureofaciens, Streptomyces lividans, and Pseudomonas fluorescens have been determined at resolutions between 1.9 A and 1.5 A. The structures of two enzymes complexed with benzoate or propionate identify the binding site for the organic acids which are required for the haloperoxidase activity. Based on these complexes and on the structure of an inactive variant, a reaction mechanism is proposed for the halogenation reaction with peroxoacid and hypohalous acid as reaction intermediates. Comparison of the structures suggests that a specific halide binding site is absent in the enzymes but that hydrophobic organic compounds may fit into the active site pocket for halogenation at preferential sites.
About this Structure
1A8S is a Single protein structure of sequence from Pseudomonas fluorescens. Full crystallographic information is available from OCA.
Reference
Structural investigation of the cofactor-free chloroperoxidases., Hofmann B, Tolzer S, Pelletier I, Altenbuchner J, van Pee KH, Hecht HJ, J Mol Biol. 1998 Jun 19;279(4):889-900. PMID:9642069
Page seeded by OCA on Sun Mar 30 18:35:43 2008
