This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
5doz
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | ''' | + | ==Crystal structure of JamJ enoyl reductase (NADPH bound)== |
| + | <StructureSection load='5doz' size='340' side='right' caption='[[5doz]], [[Resolution|resolution]] 2.26Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5doz]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DOZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DOZ FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5dov|5dov]], [[5dp1|5dp1]], [[5dp2|5dp2]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5doz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5doz OCA], [http://pdbe.org/5doz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5doz RCSB], [http://www.ebi.ac.uk/pdbsum/5doz PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The natural product curacin A, a potent anticancer agent, contains a rare cyclopropane group. The five enzymes for cyclopropane biosynthesis are highly similar to enzymes that generate a vinyl chloride moiety in the jamaicamide natural product. The structural biology of this remarkable catalytic adaptability is probed with high-resolution crystal structures of the curacin cyclopropanase (CurF ER), an in vitro enoyl reductase (JamJ ER), and a canonical curacin enoyl reductase (CurK ER). The JamJ and CurK ERs catalyze NADPH-dependent double bond reductions typical of enoyl reductases (ERs) of the medium-chain dehydrogenase reductase (MDR) superfamily. Cyclopropane formation by CurF ER is specified by a short loop which, when transplanted to JamJ ER, confers cyclopropanase activity on the chimeric enzyme. Detection of an adduct of NADPH with the model substrate crotonyl-CoA provides indirect support for a recent proposal of a C2-ene intermediate on the reaction pathway of MDR enoyl-thioester reductases. | ||
| - | + | Structural Basis for Cyclopropanation by a Unique Enoyl-Acyl Carrier Protein Reductase.,Khare D, Hale WA, Tripathi A, Gu L, Sherman DH, Gerwick WH, Hakansson K, Smith JL Structure. 2015 Oct 24. pii: S0969-2126(15)00405-0. doi:, 10.1016/j.str.2015.09.013. PMID:26526850<ref>PMID:26526850</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 5doz" style="background-color:#fffaf0;"></div> | |
| - | + | == References == | |
| - | + | <references/> | |
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Khare, D]] | [[Category: Khare, D]] | ||
| + | [[Category: Smith, J L]] | ||
| + | [[Category: Cyclopropane]] | ||
| + | [[Category: Enoyl reductase]] | ||
| + | [[Category: Nadph]] | ||
| + | [[Category: Oxidoreductase]] | ||
| + | [[Category: Polyketide synthase]] | ||
Revision as of 19:00, 1 December 2015
Crystal structure of JamJ enoyl reductase (NADPH bound)
| |||||||||||
