1ag6
From Proteopedia
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|PDB= 1ag6 |SIZE=350|CAPTION= <scene name='initialview01'>1ag6</scene>, resolution 1.6Å | |PDB= 1ag6 |SIZE=350|CAPTION= <scene name='initialview01'>1ag6</scene>, resolution 1.6Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=CU:COPPER (II) ION'>CU</scene> | + | |LIGAND= <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ag6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ag6 OCA], [http://www.ebi.ac.uk/pdbsum/1ag6 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ag6 RCSB]</span> | ||
}} | }} | ||
| Line 25: | Line 28: | ||
[[Category: Xue, Y.]] | [[Category: Xue, Y.]] | ||
[[Category: Young, S.]] | [[Category: Young, S.]] | ||
| - | [[Category: CU]] | ||
[[Category: blue-copper protein]] | [[Category: blue-copper protein]] | ||
[[Category: electron transfer]] | [[Category: electron transfer]] | ||
[[Category: electron transport]] | [[Category: electron transport]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:39:30 2008'' |
Revision as of 15:39, 30 March 2008
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| , resolution 1.6Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
PLASTOCYANIN FROM SPINACH
Overview
The crystal structure of plastocyanin from spinach has been determined using molecular replacement, with the structure of plastocyanin from poplar as a search model. Successful crystallization was facilitated by site-directed mutagenesis in which residue Gly8 was substituted with Asp. The region around residue 8 was believed to be too mobile for the wild-type protein to form crystals despite extensive screening. The current structure represents the oxidized plastocyanin, copper (II), at low pH (approximately 4.4). In contrast to the similarity in the core region as compared to its poplar counterpart, the structure shows some significant differences in loop regions. The most notable is the large shift of the 59-61 loop where the largest shift is 3.0 A for the C(alpha) atom of Glu59. This results in different patterns of electrostatic potential around the acidic patches for the two proteins.
About this Structure
1AG6 is a Single protein structure of sequence from Spinacia oleracea. Full crystallographic information is available from OCA.
Reference
Crystal structure of spinach plastocyanin at 1.7 A resolution., Xue Y, Okvist M, Hansson O, Young S, Protein Sci. 1998 Oct;7(10):2099-105. PMID:9792096
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