This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
5c3u
From Proteopedia
(Difference between revisions)
m (Protected "5c3u" [edit=sysop:move=sysop]) |
|||
| Line 1: | Line 1: | ||
| - | ''' | + | ==Crystal structure of a fungal L-serine ammonia-lyase from Rhizomucor miehei== |
| + | <StructureSection load='5c3u' size='340' side='right' caption='[[5c3u]], [[Resolution|resolution]] 1.76Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5c3u]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C3U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5C3U FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/L-serine_ammonia-lyase L-serine ammonia-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.1.17 4.3.1.17] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5c3u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c3u OCA], [http://pdbe.org/5c3u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5c3u RCSB], [http://www.ebi.ac.uk/pdbsum/5c3u PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | l-serine ammonia-lyase, as a member of the beta-family of pyridoxal-5'-phosphate (PLP) dependent enzymes, catalyzes the conversion of l-serine (l-threonine) to pyruvate (alpha-ketobutyrate) and ammonia. The crystal structure of l-serine ammonia-lyase from Rhizomucor miehei (RmSDH) was solved at 1.76 A resolution by X-ray diffraction method. The overall structure of RmSDH had the characteristic beta-family PLP dependent enzyme fold. It consisted of two distinct domains, both of which show the typical open twisted alpha/beta structure. A PLP cofactor was located in the crevice between the two domains, which was attached to Lys52 by a Schiff-base linkage. Unique residue substitutions (Gly78, Pro79, Ser146, Ser147 and Thr312) were discovered at the catalytic site of RmSDH by comparison of structures of RmSDH and other reported eukaryotic l-serine ammonia-lyases. Optimal pH and temperature of the purified RmSDH were 7.5 and 40 degrees C, respectively. It was stable in the pH range of 7.0-9.0 and at temperatures below 40 degrees C. This is the first crystal structure of a fungal l-serine ammonia-lyase. It will be useful to study the catalytic mechanism of beta-elimination enzymes and will provide a basis for further enzyme engineering. | ||
| - | + | Crystal structure and characterization of a novel l-serine ammonia-lyase from Rhizomucor miehei.,Qin Z, Yan Q, Ma Q, Jiang Z Biochem Biophys Res Commun. 2015 Oct 23;466(3):431-7. doi:, 10.1016/j.bbrc.2015.09.043. Epub 2015 Sep 11. PMID:26367174<ref>PMID:26367174</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 5c3u" style="background-color:#fffaf0;"></div> | |
| - | + | == References == | |
| - | [[Category: | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: L-serine ammonia-lyase]] | ||
| + | [[Category: Qiaojuan, Y]] | ||
[[Category: Shaoqing, Y]] | [[Category: Shaoqing, Y]] | ||
[[Category: Zhen, Q]] | [[Category: Zhen, Q]] | ||
| - | [[Category: | + | [[Category: Zhengqiang, J]] |
| + | [[Category: Beta-family plp dependent enzyme]] | ||
| + | [[Category: Lyase]] | ||
Revision as of 13:38, 9 December 2015
Crystal structure of a fungal L-serine ammonia-lyase from Rhizomucor miehei
| |||||||||||
