1b8h
From Proteopedia
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|LIGAND= | |LIGAND= | ||
|ACTIVITY= | |ACTIVITY= | ||
| - | |GENE= GENE 45 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= | + | |GENE= GENE 45 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=12353 Enterobacteria phage RB69]) |
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b8h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b8h OCA], [http://www.ebi.ac.uk/pdbsum/1b8h PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1b8h RCSB]</span> | ||
}} | }} | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1B8H is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ | + | 1B8H is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Enterobacteria_phage_rb69 Enterobacteria phage rb69]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B8H OCA]. |
==Reference== | ==Reference== | ||
Building a replisome from interacting pieces: sliding clamp complexed to a peptide from DNA polymerase and a polymerase editing complex., Shamoo Y, Steitz TA, Cell. 1999 Oct 15;99(2):155-66. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10535734 10535734] | Building a replisome from interacting pieces: sliding clamp complexed to a peptide from DNA polymerase and a polymerase editing complex., Shamoo Y, Steitz TA, Cell. 1999 Oct 15;99(2):155-66. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10535734 10535734] | ||
| - | [[Category: Enterobacteria phage | + | [[Category: Enterobacteria phage rb69]] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Shamoo, Y.]] | [[Category: Shamoo, Y.]] | ||
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[[Category: sliding clamp]] | [[Category: sliding clamp]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:55:44 2008'' |
Revision as of 15:55, 30 March 2008
| |||||||
| , resolution 3.00Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | GENE 45 (Enterobacteria phage RB69) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
SLIDING CLAMP, DNA POLYMERASE
Overview
We have solved the crystal structures of the bacteriophage RB69 sliding clamp, its complex with a peptide essential for DNA polymerase interactions, and the DNA polymerase complexed with primer-template DNA. The editing complex structure shows a partially melted duplex DNA exiting from the exonuclease domain at an unexpected angle and significant changes in the protein structure. The clamp complex shows the C-terminal 11 residues of polymerase bound in a hydrophobic pocket, and it allows docking of the editing and clamp structures together. The peptide binds to the sliding clamp at a position identical to that of a replication inhibitor peptide bound to PCNA, suggesting that the replication inhibitor protein p21CIP1 functions by competing with eukaryotic polymerases for the same binding pocket on the clamp.
About this Structure
1B8H is a Protein complex structure of sequences from Enterobacteria phage rb69. Full crystallographic information is available from OCA.
Reference
Building a replisome from interacting pieces: sliding clamp complexed to a peptide from DNA polymerase and a polymerase editing complex., Shamoo Y, Steitz TA, Cell. 1999 Oct 15;99(2):155-66. PMID:10535734
Page seeded by OCA on Sun Mar 30 18:55:44 2008
