DNA polymerase
From Proteopedia
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| - | {{STRUCTURE_1taq| PDB=1taq | SIZE=350| SCENE=DNA_polymerase/Cv/1 |right|CAPTION=Family A DNA polymerase complex with octylglucoside [[1taq]] }} | + | {{STRUCTURE_1taq| PDB=1taq | SIZE=350| SCENE=DNA_polymerase/Cv/1 |right|CAPTION=Family A DNA polymerase I complex with octylglucoside [[1taq]] }} |
[[DNA polymerase]] '''(Dpo)''' reads a [[DNA|DNA strand]] and catalyzes the synthesis of its complementary strand. Dpo's are divided into 7 families according to their sequence homology and 3D structure similarities.<ref>PMID:10364165</ref> The families are:<br /> | [[DNA polymerase]] '''(Dpo)''' reads a [[DNA|DNA strand]] and catalyzes the synthesis of its complementary strand. Dpo's are divided into 7 families according to their sequence homology and 3D structure similarities.<ref>PMID:10364165</ref> The families are:<br /> | ||
Revision as of 12:23, 3 January 2016
DNA polymerase (Dpo) reads a DNA strand and catalyzes the synthesis of its complementary strand. Dpo's are divided into 7 families according to their sequence homology and 3D structure similarities.[1] The families are:
- Family A - replicative or repair Dpo.
- Family B - replicative Dpo involved in processing DNA replication during cell division (includes eukaryotic Dpo α,δ,ε).
- Family C - major replicative Dpo in bacteria (includes Dpo II, III, IV).
- Family D - replicative. Present in archaea.
- Family X - eukaryotic repair Dpo (includes Dpo β,λ,σ,μ and terminal deoxynucleotidyl transferase).
- Family Y - replicative of damaged DNA (includes eukaryotic η,ι,κ).
- Family RT - reverse transcriptase. See Reverse transcriptase.
Some Dpo terminology:
Dpo sliding clamp is made of the complex of Dpo and Proliferating Cell Nuclear Antigen (PCNA) which encircles it.
The BRCT domain in Dpo is the C-terminal domain of breast cancer susceptibility protein.
Klenow fragment is a large Dpo fragment produced upon cleavage of Dpo by subtilisin.
In the E. coli, the EcDpo III subunits β, γ, δ, δ' are named clamp loader. This complex assembles the β subunit sliding clamp unto the DNA.
Prokaryotic DNA polymerases:
- DNA polymerase I: involved in excision repair. Major polymerase in E. coli. For more details see DNA Polymerase I
- DNA polymerase II: active in replication forks.
- DNA polymerase III: involved in DNA replication. For more details see Polymerase III homoenzyme beta subunit and Alpha Subunit of Thermus aquaticus DNA Polymerase III.
- DNA polymerase IV: involved in non-targeted mutagenesis.
- DNA polymerase V: involved in translesion synthesis.
Eukaryotic DNA polymerases:
- Pol α,β,γ,δ,ε,ζ,η,ι,κ
- Rev1 is a Dpo involved in replication over DNA lesions.
- Terminal deoxynucleotidyl transferase (TdT) is a family X Dpo expressed in immature lymphoid cells. TdT adds nucleotides to exons during antibody gene recombination.
See also User:Karl E. Zahn/RB69 DNA polymerase (gp43)
3D Structures of DNA polymerase
Updated on 03-January-2016
References
- ↑ Steitz TA. DNA polymerases: structural diversity and common mechanisms. J Biol Chem. 1999 Jun 18;274(25):17395-8. PMID:10364165
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