DNA polymerase
From Proteopedia
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**[[4p4m]], [[4p4o]], [[4p4p]] - Dpo β + DNA + nucleotide – ''Leishmania infantum''<br /> | **[[4p4m]], [[4p4o]], [[4p4p]] - Dpo β + DNA + nucleotide – ''Leishmania infantum''<br /> | ||
**[[4ppx]] - hDpo β + spiroiminohydantoin + DNA<br /> | **[[4ppx]] - hDpo β + spiroiminohydantoin + DNA<br /> | ||
| - | **[[3q8p]], [[3q8q]], [[3lk9]], [[3mby]], [[3isb]], [[3isc]], [[1zjm]], [[1tv9]], [[1tva]], [[1bpx]], [[1bpz]], [[1zqa]], [[1zqb]], [[1zqc]], [[1zqd]], [[1zqf]], [[1zqg]], [[1zqh]], [[1zqi]], [[1zaj]], [[1zqk]], [[1zql]], [[1zqm]], [[1zqn]], [[1zqo]], [[ | + | **[[3q8p]], [[3q8q]], [[3lk9]], [[3mby]], [[3isb]], [[3isc]], [[1zjm]], [[1tv9]], [[1tva]], [[1bpx]], [[1bpz]], [[1zqa]], [[1zqb]], [[1zqc]], [[1zqd]], [[1zqf]], [[1zqg]], [[1zqh]], [[1zqi]], [[1zaj]], [[1zqk]], [[1zql]], [[1zqm]], [[1zqn]], [[1zqo]], [[1zqp]], [[1zqq]], [[1zqr]], [[1zqs]], [[1zqt]], [[1zqu]], [[1zqv]], [[1zqw]], [[1zqx]], [[1zqy]], [[1zqz]], [[7ice]], [[7icf]], [[7ici]], [[7icj]], [[7icl]], [[7icm]], [[7icp]], [[7icr]], [[7ict]], [[7icu]], [[7icv]], [[8icb]], [[8icc]], [[9icj]], [[9ick]], [[9icl]], [[9icm]], [[9icp]], [[9icw]], [[9icx]], [[9icy]], [[7icg]], [[7ich]], [[7ick]], [[7icn]], [[7ico]], [[7icq]], [[7ics]], [[1zqe]], [[4gxi]], [[4gxj]], [[4gxk]], [[4kld]], [[4kle]], [[4klf]], [[4klg]], [[4klh]], [[4kli]], [[4klj]], [[4kll]], [[4klm]], [[4klo]], [[4klp]], [[4klq]], [[4kls]], [[4klt]], [[4klu]], [[1zqj]], [[4lvs]], [[4m2y]], [[4m47]], [[4nlk]], [[4nln]], [[4nlz]], [[4nm1]], [[4nm2]], [[3gdx]], [[3rh4]], [[3rh5]], [[3rh6]], [[4tup]], [[4tuq]], [[4tur]], [[4tus]] - hDpo β + DNA<br /> |
**[[3jpn]], [[3jpo]], [[3jpp]], [[3jpq]], [[3jpr]], [[3jpt]], [[3jps]], [[3isd]], [[3c2k]], [[3c2l]], [[3c2m]], [[2pxi]], [[2iso]], [[2isp]], [[2i9g]], [[2fmp]], [[2fmq]], [[2fms]], [[1zjn]], [[1mq2]], [[1mq3]], [[1bpy]], [[8ica]], [[8ice]], [[8icf]], [[8icg]], [[8ich]], [[8ici]], [[8icj]], [[8ick]], [[8icl]], [[8icm]], [[8icn]], [[8ico]], [[8icp]], [[8icq]], [[8icr]], [[8ics]], [[8ict]], [[8icu]], [[8icv]], [[8icw]], [[8icx]], [[8icz]], [[9ice]], [[9icg]], [[9ich]], [[9ici]], [[9icq]], [[9ics]], [[9ict]], [[9icu]], [[9icv]], [[9icr]], [[9icn]], [[9ico]], [[8icy]], [[9ica]], [[9icb]], [[9icc]], [[9icf]], [[4do9]], [[4doa]], [[4dob]], [[4doc]], [[3rje]], [[3rjf]], [[3rjg]], [[3rjh]], [[3rji]], [[3rjj]], [[3rjk]], [[3tfr]], [[3tfs]], [[4mf2]], [[4mf8]], [[4mfa]], [[4mfc]], [[4mff]], [[4nxz]], [[4ny8]], [[4o5c]], [[4o5e]], [[4o5k]], [[4o9m]], [[4p2h]], [[4pgq]], [[4pgx]], [[4pha]], [[4phd]], [[4uaw]], [[4uay]], [[4uaz]], [[4ub1]], [[4ub2]], [[4ub3]], [[4ub4]], [[4ub5]], [[4ubb]], [[4ubc]] - hDpo β + DNA + nucleotide<br /> | **[[3jpn]], [[3jpo]], [[3jpp]], [[3jpq]], [[3jpr]], [[3jpt]], [[3jps]], [[3isd]], [[3c2k]], [[3c2l]], [[3c2m]], [[2pxi]], [[2iso]], [[2isp]], [[2i9g]], [[2fmp]], [[2fmq]], [[2fms]], [[1zjn]], [[1mq2]], [[1mq3]], [[1bpy]], [[8ica]], [[8ice]], [[8icf]], [[8icg]], [[8ich]], [[8ici]], [[8icj]], [[8ick]], [[8icl]], [[8icm]], [[8icn]], [[8ico]], [[8icp]], [[8icq]], [[8icr]], [[8ics]], [[8ict]], [[8icu]], [[8icv]], [[8icw]], [[8icx]], [[8icz]], [[9ice]], [[9icg]], [[9ich]], [[9ici]], [[9icq]], [[9ics]], [[9ict]], [[9icu]], [[9icv]], [[9icr]], [[9icn]], [[9ico]], [[8icy]], [[9ica]], [[9icb]], [[9icc]], [[9icf]], [[4do9]], [[4doa]], [[4dob]], [[4doc]], [[3rje]], [[3rjf]], [[3rjg]], [[3rjh]], [[3rji]], [[3rjj]], [[3rjk]], [[3tfr]], [[3tfs]], [[4mf2]], [[4mf8]], [[4mfa]], [[4mfc]], [[4mff]], [[4nxz]], [[4ny8]], [[4o5c]], [[4o5e]], [[4o5k]], [[4o9m]], [[4p2h]], [[4pgq]], [[4pgx]], [[4pha]], [[4phd]], [[4uaw]], [[4uay]], [[4uaz]], [[4ub1]], [[4ub2]], [[4ub3]], [[4ub4]], [[4ub5]], [[4ubb]], [[4ubc]] - hDpo β + DNA + nucleotide<br /> | ||
**[[2p66]], [[4f5n]] - hDpo β + DNA + nucleotide analog<br /> | **[[2p66]], [[4f5n]] - hDpo β + DNA + nucleotide analog<br /> | ||
Revision as of 17:53, 5 January 2016
DNA polymerase (Dpo) reads a DNA strand and catalyzes the synthesis of its complementary strand. Dpo's are divided into 7 families according to their sequence homology and 3D structure similarities.[1] The families are:
- Family A - replicative or repair Dpo.
- Family B - replicative Dpo involved in processing DNA replication during cell division (includes eukaryotic Dpo α,δ,ε).
- Family C - major replicative Dpo in bacteria (includes Dpo II, III, IV).
- Family D - replicative. Present in archaea.
- Family X - eukaryotic repair Dpo (includes Dpo β,λ,σ,μ and terminal deoxynucleotidyl transferase).
- Family Y - replicative of damaged DNA (includes eukaryotic η,ι,κ).
- Family RT - reverse transcriptase. See Reverse transcriptase.
Some Dpo terminology:
Dpo sliding clamp is made of the complex of Dpo and Proliferating Cell Nuclear Antigen (PCNA) which encircles it.
The BRCT domain in Dpo is the C-terminal domain of breast cancer susceptibility protein.
Klenow fragment is a large Dpo fragment produced upon cleavage of Dpo by subtilisin.
In the E. coli, the EcDpo III subunits β, γ, δ, δ' are named clamp loader. This complex assembles the β subunit sliding clamp unto the DNA.
Prokaryotic DNA polymerases:
- DNA polymerase I: involved in excision repair. Major polymerase in E. coli. For more details see DNA Polymerase I
- DNA polymerase II: active in replication forks.
- DNA polymerase III: involved in DNA replication. For more details see Polymerase III homoenzyme beta subunit and Alpha Subunit of Thermus aquaticus DNA Polymerase III.
- DNA polymerase IV: involved in non-targeted mutagenesis.
- DNA polymerase V: involved in translesion synthesis.
Eukaryotic DNA polymerases:
- Pol α,β,γ,δ,ε,ζ,η,ι,κ
- Rev1 is a Dpo involved in replication over DNA lesions.
- Terminal deoxynucleotidyl transferase (TdT) is a family X Dpo expressed in immature lymphoid cells. TdT adds nucleotides to exons during antibody gene recombination.
See also User:Karl E. Zahn/RB69 DNA polymerase (gp43)
3D Structures of DNA polymerase
Updated on 05-January-2016
References
- ↑ Steitz TA. DNA polymerases: structural diversity and common mechanisms. J Biol Chem. 1999 Jun 18;274(25):17395-8. PMID:10364165
Proteopedia Page Contributors and Editors (what is this?)
Michal Harel, Cori Damron, Alexander Berchansky, Jaime Prilusky, Emily Bray, Joel L. Sussman, David Canner
