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Avidin

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The binding affinity of biotin for the avidin <scene name='41/410356/Cv/10'>receptors</scene> is very hight or in other words, the strength of the interations between the biotin and the avidin receptors is so strong that washing the biotin-avidin complex is not enough to remove the ligand from its pocket. This is not even possible by adding more biotin molecules to the system avidin-biotin. Once a <scene name='41/410356/Cv/12'>biotin has bound a pocket in the avidin</scene>, it is almost imposible to remove it in a biologica system!
The binding affinity of biotin for the avidin <scene name='41/410356/Cv/10'>receptors</scene> is very hight or in other words, the strength of the interations between the biotin and the avidin receptors is so strong that washing the biotin-avidin complex is not enough to remove the ligand from its pocket. This is not even possible by adding more biotin molecules to the system avidin-biotin. Once a <scene name='41/410356/Cv/12'>biotin has bound a pocket in the avidin</scene>, it is almost imposible to remove it in a biologica system!
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Each monomer is an eight-stranded antiparallel <scene name='Sandbox-test/Avidin_b-barrels/1'>beta-barrel</scene>. Binding of biotin involves a highly stabilized network of polar and hydrophobic interactions.
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Each monomer is an eight-stranded antiparallel <scene name='41/410356/Cv/13'>beta-barrel</scene>. Binding of biotin involves a highly stabilized network of polar and hydrophobic interactions.
The presence of additional hydrophobic and hydrophilic groups in the binding site of avidin may account for its higher affinity constant.
The presence of additional hydrophobic and hydrophilic groups in the binding site of avidin may account for its higher affinity constant.

Revision as of 13:10, 12 January 2016

The avidin tetramer complex with biotin (PDB code 2avi)

Drag the structure with the mouse to rotate

3D structures of Avidin

Updated on 12-January-2016


Reference

  • Livnah O, Bayer EA, Wilchek M, Sussman JL. Three-dimensional structures of avidin and the avidin-biotin complex. Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):5076-80. PMID:8506353
  • Livnah O, Bayer EA, Wilchek M, Sussman JL. The structure of the complex between avidin and the dye, 2-(4'-hydroxyazobenzene) benzoic acid (HABA). FEBS Lett. 1993 Aug 9;328(1-2):165-8. PMID:8344421
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