EPSP synthase
From Proteopedia
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== Structure of EPSP Synthase == | == Structure of EPSP Synthase == | ||
<StructureSection load='1eps' size='350' side='right' caption='Structure of EPSP synthase (PDB entry [[1eps]])' scene=''> | <StructureSection load='1eps' size='350' side='right' caption='Structure of EPSP synthase (PDB entry [[1eps]])' scene=''> | ||
| + | == Function == | ||
| - | '''5-enolpyruvylshikimate 3-phosphate (EPSP) synthase''' is a key enzyme for the biosynthesis of aromatic amino acids in plants and many microbes. Consequently | + | '''5-enolpyruvylshikimate 3-phosphate (EPSP) synthase''' is a key enzyme for the biosynthesis of aromatic amino acids in plants and many microbes. Consequently. EPSP synthase catalyzes the addition of phosphoenol pyruvate (PEP) to shikimate-3-phosphate, generating 5-enolpyruvylshikimate-3-phosphate, which is a precursor for phenylalanine and tyrosine<ref>PMID:16225867</ref>. |
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| + | == Relevance == | ||
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| + | EPSP synthase is a target for drugs and herbicides. | ||
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| + | == Structural insights == | ||
The enzyme has <scene name='57/570585/Two_domains/1'>two domains</scene>, with the active site found in the interdomain cleft. There is a substantial structural change upon substrate binding, resulting in a <scene name='57/570585/Closed_formation/1'>closed formation</scene>. Glyphosate (also known as Roundup) occupies the binding site of the second substrate, phosphoenol pyruvate. | The enzyme has <scene name='57/570585/Two_domains/1'>two domains</scene>, with the active site found in the interdomain cleft. There is a substantial structural change upon substrate binding, resulting in a <scene name='57/570585/Closed_formation/1'>closed formation</scene>. Glyphosate (also known as Roundup) occupies the binding site of the second substrate, phosphoenol pyruvate. | ||
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**[[3slh]] - CbEPSP + S3P + glyphosate<br /> | **[[3slh]] - CbEPSP + S3P + glyphosate<br /> | ||
}} | }} | ||
| - | + | == References == | |
| + | <references/> | ||
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[[Category:Topic Page]] | [[Category:Topic Page]] | ||
Revision as of 21:24, 19 January 2016
Structure of EPSP Synthase
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3D structures of EPSP synthase
Updated on 19-January-2016
References
- ↑ Priestman MA, Healy ML, Funke T, Becker A, Schonbrunn E. Molecular basis for the glyphosate-insensitivity of the reaction of 5-enolpyruvylshikimate 3-phosphate synthase with shikimate. FEBS Lett. 2005 Oct 24;579(25):5773-80. PMID:16225867 doi:10.1016/j.febslet.2005.09.066
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