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1cjw

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|PDB= 1cjw |SIZE=350|CAPTION= <scene name='initialview01'>1cjw</scene>, resolution 1.80&Aring;
|PDB= 1cjw |SIZE=350|CAPTION= <scene name='initialview01'>1cjw</scene>, resolution 1.80&Aring;
|SITE=
|SITE=
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|LIGAND= <scene name='pdbligand=COT:COA-S-ACETYL TRYPTAMINE'>COT</scene>
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|LIGAND= <scene name='pdbligand=COT:COA-S-ACETYL+TRYPTAMINE'>COT</scene>
|ACTIVITY=
|ACTIVITY=
|GENE=
|GENE=
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|DOMAIN=
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cjw OCA], [http://www.ebi.ac.uk/pdbsum/1cjw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1cjw RCSB]</span>
}}
}}
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[[Category: Klein, D C.]]
[[Category: Klein, D C.]]
[[Category: Namboodiri, M A.A.]]
[[Category: Namboodiri, M A.A.]]
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[[Category: COT]]
 
[[Category: n-acetyl transferase]]
[[Category: n-acetyl transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:25:19 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:23:07 2008''

Revision as of 16:23, 30 March 2008


PDB ID 1cjw

Drag the structure with the mouse to rotate
, resolution 1.80Å
Ligands:
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



SEROTONIN N-ACETYLTRANFERASE COMPLEXED WITH A BISUBSTRATE ANALOG


Overview

Serotonin N-acetyltransferase, a member of the GNAT acetyltransferase superfamily, is the penultimate enzyme in the conversion of serotonin to melatonin, the circadian neurohormone. Comparison of the structures of the substrate-free enzyme and the complex with a bisubstrate analog, coenzyme A-S-acetyltryptamine, demonstrates that acetyl coenzyme A (AcCoA) binding is accompanied by a large conformational change that in turn leads to the formation of the serotonin-binding site. The structure of the complex also provides insight into how the enzyme may facilitate acetyl transfer. A water-filled channel leading from the active site to the surface provides a pathway for proton removal following amine deprotonation. Furthermore, structural and mutagenesis results indicate an important role for Tyr-168 in catalysis.

About this Structure

1CJW is a Single protein structure of sequence from Ovis aries. Full crystallographic information is available from OCA.

Reference

The structural basis of ordered substrate binding by serotonin N-acetyltransferase: enzyme complex at 1.8 A resolution with a bisubstrate analog., Hickman AB, Namboodiri MA, Klein DC, Dyda F, Cell. 1999 Apr 30;97(3):361-9. PMID:10319816

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