This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1cxk
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 1cxk |SIZE=350|CAPTION= <scene name='initialview01'>1cxk</scene>, resolution 2.090Å | |PDB= 1cxk |SIZE=350|CAPTION= <scene name='initialview01'>1cxk</scene>, resolution 2.090Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | + | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:GLUCOSE'>GLC</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Cyclomaltodextrin_glucanotransferase Cyclomaltodextrin glucanotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.19 2.4.1.19] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Cyclomaltodextrin_glucanotransferase Cyclomaltodextrin glucanotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.19 2.4.1.19] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cxk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cxk OCA], [http://www.ebi.ac.uk/pdbsum/1cxk PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1cxk RCSB]</span> | ||
}} | }} | ||
| Line 26: | Line 29: | ||
[[Category: Kalk, K H.]] | [[Category: Kalk, K H.]] | ||
[[Category: Uitdehaag, J C.M.]] | [[Category: Uitdehaag, J C.M.]] | ||
| - | [[Category: CA]] | ||
[[Category: alpha-amylase family]] | [[Category: alpha-amylase family]] | ||
[[Category: glycosyl hydrolase family 13]] | [[Category: glycosyl hydrolase family 13]] | ||
| Line 32: | Line 34: | ||
[[Category: substrate complex]] | [[Category: substrate complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:30:31 2008'' |
Revision as of 16:30, 30 March 2008
| |||||||
| , resolution 2.090Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Activity: | Cyclomaltodextrin glucanotransferase, with EC number 2.4.1.19 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
COMPLEX BETWEEN A MALTONONAOSE SUBSTRATE AND BACILLUS CIRCULANS STRAIN 251 CGTASE E257Q/D229N
Overview
Cyclodextrin glycosyltransferase (CGTase) is an enzyme of the alpha-amylase family, which uses a double displacement mechanism to process alpha-linked glucose polymers. We have determined two X-ray structures of CGTase complexes, one with an intact substrate at 2.1 A resolution, and the other with a covalently bound reaction intermediate at 1.8 A resolution. These structures give evidence for substrate distortion and the covalent character of the intermediate and for the first time show, in atomic detail, how catalysis in the alpha-amylase family proceeds by the concerted action of all active site residues.
About this Structure
1CXK is a Single protein structure of sequence from Bacillus circulans. Full crystallographic information is available from OCA.
Reference
X-ray structures along the reaction pathway of cyclodextrin glycosyltransferase elucidate catalysis in the alpha-amylase family., Uitdehaag JC, Mosi R, Kalk KH, van der Veen BA, Dijkhuizen L, Withers SG, Dijkstra BW, Nat Struct Biol. 1999 May;6(5):432-6. PMID:10331869
Page seeded by OCA on Sun Mar 30 19:30:31 2008
