1da1
From Proteopedia
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|PDB= 1da1 |SIZE=350|CAPTION= <scene name='initialview01'>1da1</scene>, resolution 2.250Å | |PDB= 1da1 |SIZE=350|CAPTION= <scene name='initialview01'>1da1</scene>, resolution 2.250Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene> | + | |LIGAND= <scene name='pdbligand=BRU:5-BROMO-2'-DEOXYURIDINE-5'-MONOPHOSPHATE'>BRU</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1da1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1da1 OCA], [http://www.ebi.ac.uk/pdbsum/1da1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1da1 RCSB]</span> | ||
}} | }} | ||
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[[Category: Kennard, O.]] | [[Category: Kennard, O.]] | ||
[[Category: Kneale, G.]] | [[Category: Kneale, G.]] | ||
| - | [[Category: MG]] | ||
[[Category: double helix]] | [[Category: double helix]] | ||
[[Category: mismatched]] | [[Category: mismatched]] | ||
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[[Category: z-dna]] | [[Category: z-dna]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:37:39 2008'' |
Revision as of 16:37, 30 March 2008
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| , resolution 2.250Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURAL CHARACTERISATION OF THE BROMOURACIL-GUANINE BASE PAIR MISMATCH IN A Z-DNA FRAGMENT
Overview
The deoxyoligonucleotide d(BrU-G-C-G-C-G) was crystallised at pH 8.2 and its structure analysed by X-ray diffraction. The unit cell, of dimensions a = 17.94, b = 30.85, c = 49.94A contains four DNA duplexes in space group P2(1)2(1)2(1). The duplexes are in the Z conformation, with four Watson-Crick G.C base pairs and two BrU.G base pairs. The structure was refined to an R factor of 0.16 at a resolution of 2.2A with 64 solvent molecules located. The BrU.G base pair mismatch is of the wobble type, with both bases in the major tautomer form and hydrogen bonds linking 0-2 of BrU with N-1 of G and N3 of BrU with 0-6 of G. There is no indication of the presence of ionised base pairs, in spite of the high pH of crystallisation. The results are discussed in terms of the mutagenic properties of 5- bromouracil.
About this Structure
1DA1 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Structural characterisation of the bromouracil.guanine base pair mismatch in a Z-DNA fragment., Brown T, Kneale G, Hunter WN, Kennard O, Nucleic Acids Res. 1986 Feb 25;14(4):1801-9. PMID:3951996
Page seeded by OCA on Sun Mar 30 19:37:39 2008
Categories: Protein complex | Brown, T. | Hunter, W N. | Kennard, O. | Kneale, G. | Double helix | Mismatched | Modified | Z-dna
