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1e4e
From Proteopedia
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|PDB= 1e4e |SIZE=350|CAPTION= <scene name='initialview01'>1e4e</scene>, resolution 2.5Å | |PDB= 1e4e |SIZE=350|CAPTION= <scene name='initialview01'>1e4e</scene>, resolution 2.5Å | ||
|SITE= <scene name='pdbsite=AC1:Phy+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:Phy+Binding+Site+For+Chain+B'>AC2</scene>, <scene name='pdbsite=AC3:Adp+Binding+Site+For+Chain+A'>AC3</scene> and <scene name='pdbsite=AC4:Adp+Binding+Site+For+Chain+B'>AC4</scene> | |SITE= <scene name='pdbsite=AC1:Phy+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:Phy+Binding+Site+For+Chain+B'>AC2</scene>, <scene name='pdbsite=AC3:Adp+Binding+Site+For+Chain+A'>AC3</scene> and <scene name='pdbsite=AC4:Adp+Binding+Site+For+Chain+B'>AC4</scene> | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PHY:1(S)-AMINOETHYL-(2-CARBOXYPROPYL)PHOSPHORYL-PHOSPHINIC+ACID'>PHY</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= VANA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1352 Enterococcus faecium]) | |GENE= VANA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1352 Enterococcus faecium]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e4e OCA], [http://www.ebi.ac.uk/pdbsum/1e4e PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1e4e RCSB]</span> | ||
}} | }} | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Roper, D I.]] | [[Category: Roper, D I.]] | ||
| - | [[Category: ADP]] | ||
| - | [[Category: GOL]] | ||
| - | [[Category: MG]] | ||
| - | [[Category: PHY]] | ||
| - | [[Category: SO4]] | ||
[[Category: antibiotic resistance]] | [[Category: antibiotic resistance]] | ||
[[Category: cell wall]] | [[Category: cell wall]] | ||
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[[Category: plasmid]] | [[Category: plasmid]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:54:54 2008'' |
Revision as of 16:54, 30 March 2008
| |||||||
| , resolution 2.5Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | , , and | ||||||
| Ligands: | , , , , | ||||||
| Gene: | VANA (Enterococcus faecium) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
D-ALANYL-D-LACATE LIGASE
Overview
d-alanine-d-lactate ligase from Enterococcus faecium BM4147 is directly responsible for the biosynthesis of alternate cell-wall precursors in bacteria, which are resistant to the glycopeptide antibiotic vancomycin. The crystal structure has been determined with data extending to 2.5-A resolution. This structure shows that the active site has unexpected interactions and is distinct from previous models for d-alanyl-d-lactate ligase mechanistic studies. It appears that the preference of the enzyme for lactate as a ligand over d-alanine could be mediated by electrostatic effects and/or a hydrogen-bonding network, which principally involve His-244. The structure of d-alanyl-d-lactate ligase provides a revised interpretation of the molecular events that lead to vancomycin resistance.
About this Structure
1E4E is a Protein complex structure of sequences from Enterococcus faecium. Full crystallographic information is available from OCA.
Reference
The molecular basis of vancomycin resistance in clinically relevant Enterococci: crystal structure of D-alanyl-D-lactate ligase (VanA)., Roper DI, Huyton T, Vagin A, Dodson G, Proc Natl Acad Sci U S A. 2000 Aug 1;97(16):8921-5. PMID:10908650
Page seeded by OCA on Sun Mar 30 19:54:54 2008
