1ebc
From Proteopedia
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|PDB= 1ebc |SIZE=350|CAPTION= <scene name='initialview01'>1ebc</scene>, resolution 1.8Å | |PDB= 1ebc |SIZE=350|CAPTION= <scene name='initialview01'>1ebc</scene>, resolution 1.8Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=CYN:CYANIDE+ION'>CYN</scene>, <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=CYN:CYANIDE+ION'>CYN</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ebc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ebc OCA], [http://www.ebi.ac.uk/pdbsum/1ebc PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ebc RCSB]</span> | ||
}} | }} | ||
| Line 27: | Line 30: | ||
[[Category: Rizzi, M.]] | [[Category: Rizzi, M.]] | ||
[[Category: Rosano, C.]] | [[Category: Rosano, C.]] | ||
| - | [[Category: CYN]] | ||
| - | [[Category: HEM]] | ||
| - | [[Category: SO4]] | ||
[[Category: globin]] | [[Category: globin]] | ||
[[Category: hemoprotein]] | [[Category: hemoprotein]] | ||
[[Category: oxigen storage]] | [[Category: oxigen storage]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:59:14 2008'' |
Revision as of 16:59, 30 March 2008
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| , resolution 1.8Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
SPERM WHALE MET-MYOGLOBIN:CYANIDE COMPLEX
Overview
The x-ray crystal structures of the cyanide derivative of Lucina pectinata monomeric hemoglobin I (L. pectinata HbI) and sperm whale (Physeter catodon) myoglobin (Mb), generally taken as reference models for monomeric hemoproteins carrying hydrogen sulfide and oxygen, respectively, have been determined at 1.9 A (R-factor = 0. 184), and 1.8 A (R-factor = 0.181) resolution, respectively, at room temperature (lambda = 1.542 A). Moreover, the x-ray crystal structure of the L. pectinata HbI:cyanide derivative has been studied at 1.4-A resolution (R-factor = 0.118) and 100 K (on a synchrotron source lambda = 0.998 A). At room temperature, the cyanide ligand is roughly parallel to the heme plane of L. pectinata HbI, being located approximately 2.5 A from the iron atom. On the other hand, the crystal structure of the L. pectinata HbI:cyanide derivative at 100 K shows that the diatomic ligand is coordinated to the iron atom in an orientation almost perpendicular to the heme (the Fe-C distance being 1.95 A), adopting a coordination geometry strictly reminescent of that observed in sperm whale Mb, at room temperature. The unusual cyanide distal site orientation observed in L. pectinata HbI, at room temperature, may reflect reduction of the heme Fe(III) atom induced by free radical species during x-ray data collection using Cu Kalpha radiation.
About this Structure
1EBC is a Single protein structure of sequence from Physeter catodon. Full crystallographic information is available from OCA.
Reference
Cyanide binding to Lucina pectinata hemoglobin I and to sperm whale myoglobin: an x-ray crystallographic study., Bolognesi M, Rosano C, Losso R, Borassi A, Rizzi M, Wittenberg JB, Boffi A, Ascenzi P, Biophys J. 1999 Aug;77(2):1093-9. PMID:10423453
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