2jkz

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[[Image:2jkz.png|left|200px]]
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==SACCHAROMYCES CEREVISIAE HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE IN COMPLEX WITH GMP (GUANOSINE 5'- MONOPHOSPHATE) (ORTHORHOMBIC CRYSTAL FORM)==
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<StructureSection load='2jkz' size='340' side='right' caption='[[2jkz]], [[Resolution|resolution]] 3.45&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2jkz]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JKZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JKZ FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=5GP:GUANOSINE-5-MONOPHOSPHATE'>5GP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2jky|2jky]], [[2xbu|2xbu]]</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hypoxanthine_phosphoribosyltransferase Hypoxanthine phosphoribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.8 2.4.2.8] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jkz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jkz OCA], [http://pdbe.org/2jkz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jkz RCSB], [http://www.ebi.ac.uk/pdbsum/2jkz PDBsum]</span></td></tr>
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</table>
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== Function ==
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[[http://www.uniprot.org/uniprot/HPRT_YEAST HPRT_YEAST]] Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway.<ref>PMID:371963</ref> <ref>PMID:6170313</ref> <ref>PMID:7035445</ref> <ref>PMID:6392474</ref> <ref>PMID:9335580</ref> <ref>PMID:11035032</ref> <ref>PMID:18245832</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jk/2jkz_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jkz ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Hypoxanthine-guanine phosphoribosyltransferase (HGPRT) is a key enzyme of the purine recycling pathway that catalyzes the conversion of 5-phospho-ribosyl-alpha-1-pyrophosphate and guanine or hypoxanthine to guanosine monophosphate (GMP) or inosine monophosphate (IMP), respectively, and pyrophosphate (PPi). We report the first crystal structure of a fungal 6-oxopurine phosphoribosyltransferase, the Saccharomyces cerevisiae HGPRT (Sc-HGPRT) in complex with GMP. The crystal structures of full length protein with (WT1) or without (WT2) sulfate that mimics the phosphate group in the PPi binding site were solved by molecular replacement using the structure of a truncated version (Delta7) solved beforehand by multiwavelength anomalous diffusion. Sc-HGPRT is a dimer and adopts the overall structure of class I phosphoribosyltransferases (PRTs) with a smaller hood domain and a short two-stranded parallel beta-sheet linking the N- to the C-terminal end. The catalytic loops in WT1 and WT2 are in an open form while in Delta7, due to an inter-subunit disulfide bridge, the catalytic loop is in either an open or closed form. The closure is concomitant with a peptide plane flipping in the PPi binding loop. Moreover, owing the flexibility of a GGGG motif conserved in fungi, all the peptide bonds of the phosphate binding loop are in trans conformation whereas in nonfungal 6-oxopurine PRTs, one cis-peptide bond is required for phosphate binding. Mutations affecting the enzyme activity or the previously characterized feedback inhibition by GMP are located at the nucleotide binding site and the dimer interface.
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{{STRUCTURE_2jkz| PDB=2jkz | SCENE= }}
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Functional significance of four successive glycine residues in the pyrophosphate binding loop of fungal 6-oxopurine phosphoribosyltransferases.,Moynie L, Giraud MF, Breton A, Boissier F, Daignan-Fornier B, Dautant A Protein Sci. 2012 Aug;21(8):1185-96. doi: 10.1002/pro.2098. Epub 2012 Jun 11. PMID:22610485<ref>PMID:22610485</ref>
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===SACCHAROMYCES CEREVISIAE HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE IN COMPLEX WITH GMP (GUANOSINE 5'- MONOPHOSPHATE) (ORTHORHOMBIC CRYSTAL FORM)===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2jkz" style="background-color:#fffaf0;"></div>
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{{ABSTRACT_PUBMED_22610485}}
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==See Also==
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*[[Phosphoribosyltransferase|Phosphoribosyltransferase]]
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==About this Structure==
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== References ==
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[[2jkz]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JKZ OCA].
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<references/>
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__TOC__
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==Reference==
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</StructureSection>
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<ref group="xtra">PMID:018245832</ref><ref group="xtra">PMID:009743633</ref><ref group="xtra">PMID:009100006</ref><references group="xtra"/>
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[[Category: Atcc 18824]]
[[Category: Hypoxanthine phosphoribosyltransferase]]
[[Category: Hypoxanthine phosphoribosyltransferase]]
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[[Category: Saccharomyces cerevisiae]]
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[[Category: Boissier, F]]
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[[Category: Boissier, F.]]
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[[Category: Breton, A]]
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[[Category: Breton, A.]]
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[[Category: Daignan-Fornier, B]]
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[[Category: Daignan-Fornier, B.]]
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[[Category: Dautant, A]]
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[[Category: Dautant, A.]]
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[[Category: Giraud, M F]]
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[[Category: Giraud, M F.]]
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[[Category: Moynie, L]]
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[[Category: Moynie, L.]]
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[[Category: Flip peptide-plane]]
[[Category: Flip peptide-plane]]
[[Category: Glycosyltransferase]]
[[Category: Glycosyltransferase]]

Current revision

SACCHAROMYCES CEREVISIAE HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE IN COMPLEX WITH GMP (GUANOSINE 5'- MONOPHOSPHATE) (ORTHORHOMBIC CRYSTAL FORM)

2jkz, resolution 3.45Å

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