1eiz
From Proteopedia
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1ej0|1EJ0]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eiz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eiz OCA], [http://www.ebi.ac.uk/pdbsum/1eiz PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1eiz RCSB]</span> | ||
}} | }} | ||
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[[Category: Staker, B L.]] | [[Category: Staker, B L.]] | ||
[[Category: Zheng, F.]] | [[Category: Zheng, F.]] | ||
| - | [[Category: SAM]] | ||
[[Category: 23s ribosomal rna]] | [[Category: 23s ribosomal rna]] | ||
[[Category: adenosyl methionine]] | [[Category: adenosyl methionine]] | ||
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[[Category: methyltransferase]] | [[Category: methyltransferase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:03:29 2008'' |
Revision as of 17:03, 30 March 2008
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| , resolution 1.7Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Related: | 1EJ0
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
FTSJ RNA METHYLTRANSFERASE COMPLEXED WITH S-ADENOSYLMETHIONINE
Overview
Structural, biochemical, and genetic techniques were applied to investigate the function of FtsJ, a recently identified heat shock protein. FtsJ is well conserved, from bacteria to humans. The 1.5 A crystal structure of FtsJ in complex with its cofactor S-adenosylmethionine revealed that FtsJ has a methyltransferase fold. The molecular surface of FtsJ exposes a putative nucleic acid binding groove composed of highly conserved, positively charged residues. Substrate analysis showed that FtsJ methylates 23S rRNA within 50S ribosomal subunits in vitro and in vivo. Null mutations in ftsJ show a dramatically altered ribosome profile, a severe growth disadvantage, and a temperature-sensitive phenotype. Our results reveal an unexpected link between the heat shock response and RNA metabolism.
About this Structure
1EIZ is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
RNA methylation under heat shock control., Bugl H, Fauman EB, Staker BL, Zheng F, Kushner SR, Saper MA, Bardwell JC, Jakob U, Mol Cell. 2000 Aug;6(2):349-60. PMID:10983982
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