1evm
From Proteopedia
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|PDB= 1evm |SIZE=350|CAPTION= <scene name='initialview01'>1evm</scene> | |PDB= 1evm |SIZE=350|CAPTION= <scene name='initialview01'>1evm</scene> | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1evm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1evm OCA], [http://www.ebi.ac.uk/pdbsum/1evm PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1evm RCSB]</span> | ||
}} | }} | ||
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[[Category: nmr structure]] | [[Category: nmr structure]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:10:38 2008'' |
Revision as of 17:10, 30 March 2008
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| Ligands: | , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
NMR OBSERVATION OF A-TETRAD
Overview
The structure of the telomeric DNA has been a subject of extensive investigation in recent years due to the realization that it has important functional roles to play in vivo and the observations that truncated telomeric sequences exhibit a great variety of 3D structures in aqueous solutions. In this context, we describe here NMR structural studies on two truncated human telomeric DNA sequences, d-AG(3)T and d-TAG(3)T in solutions containing K(+)ions. The G(3)stretches in both the oligonucleotides were seen to form parallel-stranded quadruplexes. However, the AG(3)segment as a whole, had different structural characteristics. The structure of d-AG(3)T revealed the formation of a novel A-tetrad, which was not seen in d-TAG(3)T. The A's in the tetrad had syn glycosidic conformation as opposed to the anti conformation of the G's in the G-tetrads. The A-tetrad stacked well over the adjacent G-tetrad and the twist angle at this step was smaller in d-AG(3)T than in d-TAG(3)T. These observations are expected to be significant from the point of view of structural diversity and recognition in telomeres.
About this Structure
1EVM is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
NMR studies on truncated sequences of human telomeric DNA: observation of a novel A-tetrad., Patel PK, Koti AS, Hosur RV, Nucleic Acids Res. 1999 Oct 1;27(19):3836-43. PMID:10481022
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