1f0k
From Proteopedia
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|PDB= 1f0k |SIZE=350|CAPTION= <scene name='initialview01'>1f0k</scene>, resolution 1.9Å | |PDB= 1f0k |SIZE=350|CAPTION= <scene name='initialview01'>1f0k</scene>, resolution 1.9Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | + | |LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1f0k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f0k OCA], [http://www.ebi.ac.uk/pdbsum/1f0k PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1f0k RCSB]</span> | ||
}} | }} | ||
| Line 26: | Line 29: | ||
[[Category: Walker, D.]] | [[Category: Walker, D.]] | ||
[[Category: Walker, S.]] | [[Category: Walker, S.]] | ||
| - | [[Category: SO4]] | ||
[[Category: rossmann fold]] | [[Category: rossmann fold]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:13:22 2008'' |
Revision as of 17:13, 30 March 2008
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| , resolution 1.9Å | |||||||
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| Ligands: | |||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
THE 1.9 ANGSTROM CRYSTAL STRUCTURE OF E. COLI MURG
Overview
The 1.9 A X-ray structure of a membrane-associated glycosyltransferase involved in peptidoglycan biosynthesis is reported. This enzyme, MurG, contains two alpha/beta open sheet domains separated by a deep cleft. Structural analysis suggests that the C-terminal domain contains the UDP-GlcNAc binding site while the N-terminal domain contains the acceptor binding site and likely membrane association site. Combined with sequence data from other MurG homologs, this structure provides insight into the residues that are important in substrate binding and catalysis. We have also noted that a conserved region found in many UDP-sugar transferases maps to a beta/alpha/beta/alpha supersecondary structural motif in the donor binding region of MurG, an observation that may be helpful in glycosyltransferase structure prediction. The identification of a conserved structural motif involved in donor binding in different UDP-sugar transferases also suggests that it may be possible to identify--and perhaps alter--the residues that help determine donor specificity.
About this Structure
1F0K is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
The 1.9 A crystal structure of Escherichia coli MurG, a membrane-associated glycosyltransferase involved in peptidoglycan biosynthesis., Ha S, Walker D, Shi Y, Walker S, Protein Sci. 2000 Jun;9(6):1045-52. PMID:10892798
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