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1ufj

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[[Image:1ufj.gif|left|200px]]
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==Crystal Structure of an Artificial Metalloprotein:Fe(III)(3,3'-Me2-salophen)/apo-A71G Myoglobin==
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<StructureSection load='1ufj' size='340' side='right' caption='[[1ufj]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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{{Structure
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== Structural highlights ==
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|PDB= 1ufj |SIZE=350|CAPTION= <scene name='initialview01'>1ufj</scene>, resolution 1.60&Aring;
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<table><tr><td colspan='2'>[[1ufj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Phycd Phycd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UFJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UFJ FirstGlance]. <br>
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|SITE=
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CZM:N,N-BIS-(2-HYDROXY-3-METHYL-BENZYLIDENE)-BENZENE-1,2-DIAMINE'>CZM</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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|LIGAND= <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene> and <scene name='pdbligand=CZM:'N,N'-BIS-(2-HYDROXY-3-METHYL-BENZYLIDENE)-BENZENE-1,2-DIAMINE''>CZM</scene>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ufj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ufj OCA], [http://pdbe.org/1ufj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ufj RCSB], [http://www.ebi.ac.uk/pdbsum/1ufj PDBsum]</span></td></tr>
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|ACTIVITY=
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</table>
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|GENE=
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== Function ==
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}}
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[[http://www.uniprot.org/uniprot/MYG_PHYMC MYG_PHYMC]] Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.
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== Evolutionary Conservation ==
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'''Crystal Structure of an Artificial Metalloprotein:Fe(III)(3,3'-Me2-salophen)/apo-A71G Myoglobin'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uf/1ufj_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ufj ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
Apo-myoglobin (apo-Mb) and apo-A71GMb were successfully reconstituted with FeIII(salophen) (1) (salophen = N,N'-bis(salicylidene)-1,2-phenilenediamine), Fe(III)(3,3'-Me2-salophen) (2), and FeIII(5,5'-t-Bu2-salophen) (3). The crystal structure of 2.apo-A71GMb shows the tight binding of the complex in the Mb cavity, while in wild-type apo-Mb it is highly disordered due to the steric repulsion with Ala71. Furthermore, the structure of 2.apo-A71GMb suggests a possible accommodation of a small substrate in the cavity. In fact, the cyanide association rate constant of 2.apo-A71GMb is 216-fold larger compared to that of 2.apo-Mb. These results provide us principles for the noncovalent fixation of synthetic metal cofactors at the desired positions in protein matrixes.
Apo-myoglobin (apo-Mb) and apo-A71GMb were successfully reconstituted with FeIII(salophen) (1) (salophen = N,N'-bis(salicylidene)-1,2-phenilenediamine), Fe(III)(3,3'-Me2-salophen) (2), and FeIII(5,5'-t-Bu2-salophen) (3). The crystal structure of 2.apo-A71GMb shows the tight binding of the complex in the Mb cavity, while in wild-type apo-Mb it is highly disordered due to the steric repulsion with Ala71. Furthermore, the structure of 2.apo-A71GMb suggests a possible accommodation of a small substrate in the cavity. In fact, the cyanide association rate constant of 2.apo-A71GMb is 216-fold larger compared to that of 2.apo-Mb. These results provide us principles for the noncovalent fixation of synthetic metal cofactors at the desired positions in protein matrixes.
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==About this Structure==
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Crystal structures of artificial metalloproteins: tight binding of FeIII(Schiff-Base) by mutation of Ala71 to Gly in apo-myoglobin.,Ueno T, Ohashi M, Kono M, Kondo K, Suzuki A, Yamane T, Watanabe Y Inorg Chem. 2004 May 3;43(9):2852-8. PMID:15106972<ref>PMID:15106972</ref>
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1UFJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Physeter_catodon Physeter catodon]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UFJ OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structures of artificial metalloproteins: tight binding of FeIII(Schiff-Base) by mutation of Ala71 to Gly in apo-myoglobin., Ueno T, Ohashi M, Kono M, Kondo K, Suzuki A, Yamane T, Watanabe Y, Inorg Chem. 2004 May 3;43(9):2852-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15106972 15106972]
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</div>
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[[Category: Physeter catodon]]
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<div class="pdbe-citations 1ufj" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Kondo, K.]]
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[[Category: Kono, M.]]
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[[Category: Ohashi, M.]]
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[[Category: Suzuki, A.]]
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[[Category: Ueno, T.]]
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[[Category: Watanabe, Y.]]
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[[Category: Yamane, T.]]
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[[Category: CZM]]
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[[Category: FE]]
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[[Category: PO4]]
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[[Category: iron]]
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[[Category: metalloprotein]]
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[[Category: myoglobin]]
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[[Category: salophen]]
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[[Category: schiff base]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:31:23 2008''
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==See Also==
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*[[Myoglobin|Myoglobin]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Phycd]]
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[[Category: Kondo, K]]
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[[Category: Kono, M]]
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[[Category: Ohashi, M]]
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[[Category: Suzuki, A]]
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[[Category: Ueno, T]]
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[[Category: Watanabe, Y]]
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[[Category: Yamane, T]]
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[[Category: Iron]]
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[[Category: Metalloprotein]]
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[[Category: Myoglobin]]
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[[Category: Oxygen storage-transport complex]]
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[[Category: Salophen]]
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[[Category: Schiff base]]

Current revision

Crystal Structure of an Artificial Metalloprotein:Fe(III)(3,3'-Me2-salophen)/apo-A71G Myoglobin

1ufj, resolution 1.60Å

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