This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
Sandbox Wabash 13
From Proteopedia
(Difference between revisions)
| Line 2: | Line 2: | ||
==Distinguishing between the active site and a nearby dicarboxylic acid binding site in Fumarase== | ==Distinguishing between the active site and a nearby dicarboxylic acid binding site in Fumarase== | ||
| - | In early studies, two carboxylic acid binding sites, subsequently named A- and B-, were observed in the wild type crystal structure of fumarase C in ''E. Coli.'' The structures of these two sites was considerably different as site A contained atom of three of the four subunits of fumarase C whereas site B contained only atoms from a single subunit of the tetramer. | + | In early studies, two carboxylic acid binding sites, subsequently named A- and B-, were observed in the wild type crystal structure of fumarase C in ''E. Coli.'' The structures of these two sites was considerably different as site A contained atom of three of the four subunits of fumarase C whereas site B contained only atoms from a single subunit of the tetramer. While it was heavily believed that the carboxylic acid binding site A- was the location of the fumarase active site, it could not be verified without first mutating both active sites in order to determine the activity of the reactions <ref>PMID:9098893</references> |
== Function == | == Function == | ||
Revision as of 16:56, 28 February 2016
| |||||||||||
