Sandbox Wabash 23 Fumarase
From Proteopedia
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==Determination of the active site Fumarase== | ==Determination of the active site Fumarase== | ||
<StructureSection load='1yfe' size='340' side='right' caption='Caption for this structure' scene=''> | <StructureSection load='1yfe' size='340' side='right' caption='Caption for this structure' scene=''> | ||
| - | Benjamin Washer | + | Benjamin Washer <ref>PMID:9098893</ref> |
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== Function == | == Function == | ||
Fumarase C is an enzyme found in ''E. coli'' (EfumC) that is similar to the cytosolic and mitochondrial enzymes found in eukaryotic cells and is used to catalyze the hydration/dehydration reaction between L-malate and fumarate. Because of its relative simplicity, homology with important eukaryotic enzymes and important metabolic influence, fumarase has been the object of much study. However, upon analysis of fumarase, it was found that there were two "active" sites and much debate has arisen regarding which is the most influential or "true" one. This article describes the debate and the process used to determine the true active site. | Fumarase C is an enzyme found in ''E. coli'' (EfumC) that is similar to the cytosolic and mitochondrial enzymes found in eukaryotic cells and is used to catalyze the hydration/dehydration reaction between L-malate and fumarate. Because of its relative simplicity, homology with important eukaryotic enzymes and important metabolic influence, fumarase has been the object of much study. However, upon analysis of fumarase, it was found that there were two "active" sites and much debate has arisen regarding which is the most influential or "true" one. This article describes the debate and the process used to determine the true active site. | ||
Revision as of 22:02, 29 February 2016
Determination of the active site Fumarase
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References
Mutations of Fumarase that Distinguish Between the Active Site and a Nearby Dicarboxylic Acid Binding Site [2] JSmol in Proteopedia [3] Jmol [4] to the rescue
