1gmm
From Proteopedia
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|PDB= 1gmm |SIZE=350|CAPTION= <scene name='initialview01'>1gmm</scene>, resolution 2.0Å | |PDB= 1gmm |SIZE=350|CAPTION= <scene name='initialview01'>1gmm</scene>, resolution 2.0Å | ||
|SITE= <scene name='pdbsite=CA1:Ca+Binding+Site+For+Chain+A'>CA1</scene> | |SITE= <scene name='pdbsite=CA1:Ca+Binding+Site+For+Chain+A'>CA1</scene> | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gmm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gmm OCA], [http://www.ebi.ac.uk/pdbsum/1gmm PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1gmm RCSB]</span> | ||
}} | }} | ||
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[[Category: Mosbah, A.]] | [[Category: Mosbah, A.]] | ||
[[Category: Zamboni, V.]] | [[Category: Zamboni, V.]] | ||
| - | [[Category: CA]] | ||
| - | [[Category: NA]] | ||
| - | [[Category: SO4]] | ||
[[Category: carbohydrate binding module]] | [[Category: carbohydrate binding module]] | ||
[[Category: cbm family 6]] | [[Category: cbm family 6]] | ||
[[Category: xylan binding]] | [[Category: xylan binding]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:47:07 2008'' |
Revision as of 17:47, 30 March 2008
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| , resolution 2.0Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | |||||||
| Ligands: | , , | ||||||
| Activity: | Endo-1,4-beta-xylanase, with EC number 3.2.1.8 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CARBOHYDRATE BINDING MODULE CBM6 FROM XYLANASE U CLOSTRIDIUM THERMOCELLUM
Overview
Polysaccharide-degrading enzymes are generally modular proteins that contain non-catalytic carbohydrate-binding modules (CBMs), which potentiate the activity of the catalytic module. CBMs have been grouped into sequence-based families, and three-dimensional structural data are available for half of these families. Clostridium thermocellum xylanase 11A is a modular enzyme that contains a CBM from family 6 (CBM6), for which no structural data are available. We have determined the crystal structure of this module to a resolution of 2.1 A. The protein is a beta-sandwich that contains two potential ligand-binding clefts designated cleft A and B. The CBM interacts primarily with xylan, and NMR spectroscopy coupled with site-directed mutagenesis identified cleft A, containing Trp-92, Tyr-34, and Asn-120, as the ligand-binding site. The overall fold of CBM6 is similar to proteins in CBM families 4 and 22, although surprisingly the ligand-binding site in CBM4 and CBM22 is equivalent to cleft B in CBM6. These structural data define a superfamily of CBMs, comprising CBM4, CBM6, and CBM22, and demonstrate that, although CBMs have evolved from a relatively small number of ancestors, the structural elements involved in ligand recognition have been assembled at different locations on the ancestral scaffold.
About this Structure
1GMM is a Single protein structure of sequence from Clostridium thermocellum. Full crystallographic information is available from OCA.
Reference
The location of the ligand-binding site of carbohydrate-binding modules that have evolved from a common sequence is not conserved., Czjzek M, Bolam DN, Mosbah A, Allouch J, Fontes CM, Ferreira LM, Bornet O, Zamboni V, Darbon H, Smith NL, Black GW, Henrissat B, Gilbert HJ, J Biol Chem. 2001 Dec 21;276(51):48580-7. Epub 2001 Oct 22. PMID:11673472
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