This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
Sandbox Wabash3
From Proteopedia
(Difference between revisions)
| Line 4: | Line 4: | ||
Kenton Hicks, Kyle Stucker, Allen Betts | Kenton Hicks, Kyle Stucker, Allen Betts | ||
| - | Trypsin is serine protease which catalyzes the hydrolysis of peptide bonds of a substrate via an acylation reaction and a deacylation reaction. In the first (acylation) reaction, the nucleophilic serine attacks the substrate scissile bond, forming a tetrahedral intermediate and then a covalent acyl-enzyme with the release of the C-terminal fragment. In the second (deacylation) reaction, a water molecule attacks the acyl-enzyme, leading to a second tetrahedral intermediate followed by release of the N-terminal fragment. The specificity of substrates is determined by the structure of its specificity pocket, which contains Ser 195, His 57, and Asp 102. These three residues make up the <scene name='72/725340/Catalytic_triad/1'>Catalytic Triad</scene | + | Trypsin is serine protease which catalyzes the hydrolysis of peptide bonds of a substrate via an acylation reaction and a deacylation reaction. In the first (acylation) reaction, the nucleophilic serine attacks the substrate scissile bond, forming a tetrahedral intermediate and then a covalent acyl-enzyme with the release of the C-terminal fragment. In the second (deacylation) reaction, a water molecule attacks the acyl-enzyme, leading to a second tetrahedral intermediate followed by release of the N-terminal fragment. The specificity of substrates is determined by the structure of its specificity pocket, which contains Ser 195, His 57, and Asp 102. These three residues make up the <scene name='72/725340/Catalytic_triad/1'>Catalytic Triad</scene> |
== <ref>PMID:16636277</ref> == | == <ref>PMID:16636277</ref> == | ||
Revision as of 18:17, 9 March 2016
Mechanism of Trypsin
| |||||||||||
