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5hmj

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== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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The platinum anti-cancer agents cisplatin and carboplatin bind to the histidine 15 residue in the model protein hen egg white lysozyme. By using temperatures either side of the protein glass transition state ( approximately 180 K), several platinum binding modes are seen and show that not all these platinum modes are stable. In particular, the mean square displacement vibration amplitudes of the cisplatin and of the histidine to which it is bound are analysed in detail. As well as the multiple platinum peaks, the electron density for the His-15 side chain is weak to absent at 150 K and 200 K, which points to the imidazole ring of the His side chain sampling multiple positions. Most interestingly, the His-15 imidazole becomes more ordered at room temperature.
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A re-refinement of 4xan, hen egg-white lysozyme (HEWL) with carboplatin crystallized in NaBr solution, has been made and is published here as an addendum to Tanley et al. [(2014), Acta Cryst. F70, 1135-1142]. This follows a previous re-refinement and PDB deposition (4yem) by Shabalin et al. [(2015), Acta Cryst. D71, 1965-1979]. The critical evaluation of the original PDB deposition (4xan), and the subsequent critical examination of the re-refined structure (4yem), has led to an improved model (PDB code 5hmj).
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Structural dynamics of cisplatin binding to histidine in a protein.,Tanley SW, Helliwell JR Struct Dyn. 2014 Jun 17;1(3):034701. doi: 10.1063/1.4883975. eCollection 2014, May. PMID:26798779<ref>PMID:26798779</ref>
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Re-refinement of 4xan: hen egg-white lysozyme with carboplatin in sodium bromide solution.,Tanley SW, Schreurs AM, Kroon-Batenburg LM, Helliwell JR Acta Crystallogr F Struct Biol Commun. 2016 Mar 1;72(Pt 3):251-252. Epub 2016 Feb, 19. PMID:26919531<ref>PMID:26919531</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

Revision as of 19:35, 9 March 2016

Re-refinement of 4xan: hen lysozyme with carboplatin in sodium bromide solution

5hmj, resolution 1.30Å

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