GMP synthase
From Proteopedia
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<StructureSection load='2vxo' size='400' side='right' caption='Structure of human GMP synthase dimer complex with xantosine 5-phosphate (stick model) and sulfate (PDB entry [[2vxo]])' scene=''> | <StructureSection load='2vxo' size='400' side='right' caption='Structure of human GMP synthase dimer complex with xantosine 5-phosphate (stick model) and sulfate (PDB entry [[2vxo]])' scene=''> | ||
== Function == | == Function == | ||
| - | '''GMP synthase''' (GMPS) catalyzes the conversion of xantosine 5’-phosphate, ATP, glutamine and water to glutamate, GMP, AMP and diphosphate. GMPS is active in purine and glutamate metabolism<ref>PMID:24462112</ref>. | + | '''GMP synthase''' (GMPS) catalyzes the conversion of xantosine 5’-phosphate (XMP), ATP, glutamine and water to glutamate, GMP, AMP and diphosphate. GMPS is active in purine and glutamate metabolism<ref>PMID:24462112</ref>. GMPS is a bifunctional two-domain enzyme with the N-terminal glutaminase dominates extracts ammonia from glutamine and the C-terminal synthetase adds amine group to XMP to produce GMP. |
== Structural highlights == | == Structural highlights == | ||
| + | Human GMPS structure contains 2 dimerization domains (D1 and D2). The active site is located between the synthetase domain and D2 domain and covered by the LID motif. The catalytic triad consists of Cys, His and Glu<ref>PMID:23816837</ref>. | ||
==3D structures of GMP synthase== | ==3D structures of GMP synthase== | ||
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[[2lxn]] – GMPS subunit A – ''Methanocaldococcus jannaschii''<br /> | [[2lxn]] – GMPS subunit A – ''Methanocaldococcus jannaschii''<br /> | ||
[[2vpi]] – hGMPS glutaminase domain – human<br /> | [[2vpi]] – hGMPS glutaminase domain – human<br /> | ||
| - | [[2vxo]] - hGMPS + | + | [[2vxo]] - hGMPS + XMP<br /> |
| - | [[2ywc]] – TtGMPS + | + | [[2ywc]] – TtGMPS + XMP<br /> |
[[2iss]] – TmGMPS subunit PDXT + PLP biosynthesis lyase PDXS<br /> | [[2iss]] – TmGMPS subunit PDXT + PLP biosynthesis lyase PDXS<br /> | ||
[[1gpm]] – GMPS + pyrophosphate + AMP – ''Escherichia coli'' | [[1gpm]] – GMPS + pyrophosphate + AMP – ''Escherichia coli'' | ||
Revision as of 12:02, 15 March 2016
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