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5how
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==X-ray crystallographic structure of an Abeta 17-36 beta-hairpin. LV(PHI)FAEDCGSNKCAII(SAR)L(ORN)V== | |
| + | <StructureSection load='5how' size='340' side='right' caption='[[5how]], [[Resolution|resolution]] 2.29Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5how]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HOW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HOW FirstGlance]. <br> | ||
| + | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ORN:L-ORNITHINE'>ORN</scene>, <scene name='pdbligand=PHI:IODO-PHENYLALANINE'>PHI</scene>, <scene name='pdbligand=SAR:SARCOSINE'>SAR</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5hox|5hox]], [[5hoy|5hoy]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5how FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5how OCA], [http://pdbe.org/5how PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5how RCSB], [http://www.ebi.ac.uk/pdbsum/5how PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | High-resolution structures of oligomers formed by the beta-amyloid peptide Abeta are needed to understand the molecular basis of Alzheimer's disease and develop therapies. This paper presents the X-ray crystallographic structures of oligomers formed by a 20-residue peptide segment derived from Abeta. The development of a peptide in which Abeta17-36 is stabilized as a beta-hairpin is described, and the X-ray crystallographic structures of oligomers it forms are reported. Two covalent constraints act in tandem to stabilize the Abeta17-36 peptide in a hairpin conformation: a delta-linked ornithine turn connecting positions 17 and 36 to create a macrocycle and an intramolecular disulfide linkage between positions 24 and 29. An N-methyl group at position 33 blocks uncontrolled aggregation. The peptide readily crystallizes as a folded beta-hairpin, which assembles hierarchically in the crystal lattice. Three beta-hairpin monomers assemble to form a triangular trimer, four trimers assemble in a tetrahedral arrangement to form a dodecamer, and five dodecamers pack together to form an annular pore. This hierarchical assembly provides a model, in which full-length Abeta transitions from an unfolded monomer to a folded beta-hairpin, which assembles to form oligomers that further pack to form an annular pore. This model may provide a better understanding of the molecular basis of Alzheimer's disease at atomic resolution. | ||
| - | + | X-ray Crystallographic Structures of a Trimer, Dodecamer, and Annular Pore Formed by an Abeta17-36 beta-Hairpin.,Kreutzer AG, Hamza IL, Spencer RK, Nowick JS J Am Chem Soc. 2016 Apr 6;138(13):4634-42. doi: 10.1021/jacs.6b01332. Epub 2016, Mar 29. PMID:26967810<ref>PMID:26967810</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Nowick, J | + | <div class="pdbe-citations 5how" style="background-color:#fffaf0;"></div> |
| - | [[Category: Spencer, R | + | == References == |
| - | [[Category: | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Kreutzer, A G]] | ||
| + | [[Category: Nowick, J S]] | ||
| + | [[Category: Spencer, R K]] | ||
| + | [[Category: Alzheimers']] | ||
| + | [[Category: Amyloid]] | ||
| + | [[Category: Beta-hairpin]] | ||
| + | [[Category: Oligomer]] | ||
| + | [[Category: Protein fibril]] | ||
Revision as of 17:17, 10 May 2016
X-ray crystallographic structure of an Abeta 17-36 beta-hairpin. LV(PHI)FAEDCGSNKCAII(SAR)L(ORN)V
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