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==PKA and Regulators==
==PKA and Regulators==
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<StructureSection load='3fhi' size='340' side='right' caption='Caption for this structure' scene=''>
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<StructureSection load='3fhi' size='340' side='right' caption='Caption for this structure' scene='58/583419/3fhi/1'>
== Structural highlights ==
== Structural highlights ==
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<scene name='58/583419/Pka_reg/1'>The PKA catalytic unit (C) crystalized with the regulatory unit (R)</scene>, pdb structure [[3fhi]]<ref>PMID:15692043</ref>, is shown with small lobe in gray, large lobe in tan and regulatory unit in light blue.
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<scene name='58/583419/Pka_reg/1'>The PKA catalytic unit (C) crystalized with the regulatory unit (R)</scene>, pdb structure [[3fhi]]<ref>PMID:15692043</ref>, is shown with small lobe in ''gray'', large lobe in ''tan'' and regulatory unit in ''light blue''.
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There is a significant distance <scene name='58/583419/Pka_reg/4'>from the residues from ''exon 1'' (labeled) to the residues which interact</scene> with the regulatory unit R (halos and ball and stick). The last three residues from exon 1 (residues # 13-15) are the only translated amino acids from first exon of pkaca that were well-structured in this crystal structure.
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There is a significant distance from the residues from '''pkaca''''s <scene name='58/583419/Pka_reg/4'> ''exon 1'' (labelled by residue index)</scene> to the residues which interact with the regulatory unit '''R''' (halos and ball and stick).
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Note that the last three residues from exon 1 (residues # 13-15) are the only translated amino acids from the first exon of pkaca that were resolved in this crystal structure.
These three residues are also <scene name='58/583419/Pka_reg/3'>distant from PKI-interacting residues (halos and ball and stick)</scene> - a second regulator/inhibitor of 'C', as well.
These three residues are also <scene name='58/583419/Pka_reg/3'>distant from PKI-interacting residues (halos and ball and stick)</scene> - a second regulator/inhibitor of 'C', as well.

Revision as of 17:00, 29 May 2016

PKA and Regulators

Caption for this structure

Drag the structure with the mouse to rotate

References

  1. Kim C, Xuong NH, Taylor SS. Crystal structure of a complex between the catalytic and regulatory (RIalpha) subunits of PKA. Science. 2005 Feb 4;307(5710):690-6. PMID:15692043 doi:307/5710/690
  2. Zheng J, Trafny EA, Knighton DR, Xuong NH, Taylor SS, Ten Eyck LF, Sowadski JM. 2.2 A refined crystal structure of the catalytic subunit of cAMP-dependent protein kinase complexed with MnATP and a peptide inhibitor. Acta Crystallogr D Biol Crystallogr. 1993 May 1;49(Pt 3):362-5. PMID:15299527 doi:10.1107/S0907444993000423

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Joseph M. Steinberger

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