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1w4r

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[[Category: type ii]]
[[Category: type ii]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 13:45:00 2007''
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 15:38:17 2007''

Revision as of 13:32, 5 November 2007


1w4r, resolution 1.83Å

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STRUCTURE OF A TYPE II THYMIDINE KINASE WITH BOUND DTTP

Overview

The structure of human cytosolic thymidine kinase in complex with its, feedback inhibitor 2'-deoxythymidine-5'-triphosphate was determined. This, structure is the first representative of the type II thymidine kinases, found in several pathogens. The structure deviates strongly from the known, structures of type I thymidine kinases such as the Herpes simplex enzyme., It contains a zinc-binding domain with four cysteines complexing a, structural zinc ion. Interestingly, the backbone atoms of the type II, enzyme bind thymine via hydrogen-bonds, in contrast to type I, where side, chains are involved. This results in a specificity difference exploited, for antiviral therapy. The presented structure will foster the development, of new drugs and prodrugs for numerous therapeutic applications.

About this Structure

1W4R is a Single protein structure of sequence from Homo sapiens with ZN, TTP and DTU as ligands. Active as Thymidine kinase, with EC number 2.7.1.21 Structure known Active Site: AC1. Full crystallographic information is available from OCA.

Reference

Structure of a type II thymidine kinase with bound dTTP., Birringer MS, Claus MT, Folkers G, Kloer DP, Schulz GE, Scapozza L, FEBS Lett. 2005 Feb 28;579(6):1376-82. PMID:15733844

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