This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
Phage integrase
From Proteopedia
| Line 1: | Line 1: | ||
| - | |||
{{STRUCTURE_1z1g| PDB=1z1g | SIZE=400| SCENE=Phage_integrase/Cv/1 |right|CAPTION= Se-Met Enterobacteria phage λ integrase:DNA tetramer complex (Holiday Junction) [[1z1g]] }} | {{STRUCTURE_1z1g| PDB=1z1g | SIZE=400| SCENE=Phage_integrase/Cv/1 |right|CAPTION= Se-Met Enterobacteria phage λ integrase:DNA tetramer complex (Holiday Junction) [[1z1g]] }} | ||
| - | '''Phage integrase''' (PIN) | + | '''Phage integrase''' (PIN) mediates recombination between DNA recognition sequences - the phage attachment site attP and the bacterial attachment site attB<ref>PMID:14687564</ref>. The '''Tyrosine family PIN''' uses a catalytic tyrosine to mediate strand cleavage while the '''Serine family PIN''' uses a serine residue. The High-Pathogenicity Island (HPI) is a genomic island essential for virulence of the family of Enterobacteria. |
| - | + | ||
| - | + | ||
== 3D Structures of phage integrase == | == 3D Structures of phage integrase == | ||
| Line 25: | Line 22: | ||
[[1aih]] - PIN catalytic core domain – Haemophilus phage HP1<br /> | [[1aih]] - PIN catalytic core domain – Haemophilus phage HP1<br /> | ||
[[2khv]], [[2kj5]] – PIN residues 102-199 – ''Nitrosospira multiformis'' - NMR | [[2khv]], [[2kj5]] – PIN residues 102-199 – ''Nitrosospira multiformis'' - NMR | ||
| + | |||
| + | == References == | ||
| + | <references/> | ||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||
Revision as of 09:28, 22 June 2016
Phage integrase (PIN) mediates recombination between DNA recognition sequences - the phage attachment site attP and the bacterial attachment site attB[1]. The Tyrosine family PIN uses a catalytic tyrosine to mediate strand cleavage while the Serine family PIN uses a serine residue. The High-Pathogenicity Island (HPI) is a genomic island essential for virulence of the family of Enterobacteria.
3D Structures of phage integrase
Update June 2011
3nrw – PIN/site-specific recombinase N-terminal – Haloarcula marismortui
3jtz – PIN arm-type binding domain – Yersinia pestis
3ju0 – HPI PIN arm-type binding domain – Pectobacterium atrosepticum
2kkp – PIN SAM-like domain – Moorella thermoacetica – NMR
2kkv – PIN fragment – Salmonella enterica – NMR
2khq - PIN fragment – Staphylococcus saprophyticus – NMR
2wcc – λPIN DBD+ DNA – Enterobacteria phage λ - NMR
1kjk – λPIN N-terminal - NMR
2oxo, 1z19, 1z1b - λPIN core binding domain + DNA
1ae9 - λPIN catalytic core domain (mutant)
1z1g – λPIN + DNA (Holliday junction)
1p7d – λPIN fragment + DNA
3bvp – PIN catalytic core domain – Lactococcus phage TP101-1
1aih - PIN catalytic core domain – Haemophilus phage HP1
2khv, 2kj5 – PIN residues 102-199 – Nitrosospira multiformis - NMR
References
- ↑ Groth AC, Calos MP. Phage integrases: biology and applications. J Mol Biol. 2004 Jan 16;335(3):667-78. PMID:14687564 doi:http://dx.doi.org/10.1016/S0022283603013561
