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5ijs
From Proteopedia
(Difference between revisions)
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ijs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ijs OCA], [http://pdbe.org/5ijs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ijs RCSB], [http://www.ebi.ac.uk/pdbsum/5ijs PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ijs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ijs OCA], [http://pdbe.org/5ijs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ijs RCSB], [http://www.ebi.ac.uk/pdbsum/5ijs PDBsum]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Autotaxin (ATX) is a secreted phosphodiesterase that produces the signalling lipid lysophosphatidic acid (LPA). The bimetallic active site of ATX is structurally related to the alkaline phosphatase superfamily. Here, we present a new crystal structure of ATX in complex with orthovanadate (ATX-VO5), which binds the Ogamma nucleophile of Thr209 and adopts a trigonal bipyramidal conformation, following the nucleophile attack onto the substrate. We have now a portfolio of ATX structures we discuss as intermediates of the catalytic mechanism: the new ATX-VO5 structure; a unique structure where the nucleophile Thr209 is phosphorylated (ATX-pThr). Comparing these to a complex with the LPA product (ATX-LPA) and with a complex with a phosphate ion (ATX-PO4), that represent the Michaelis complex of the reaction, we observe movements of Thr209, changes in the relative displacement of the zinc ions, and a water molecule that likely fulfils the second nucleophilic attack. We propose that ATX follows the associative two-step in-line displacement mechanism. | ||
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| + | Structural snapshots of the catalytic cycle of the phosphodiesterase Autotaxin.,Hausmann J, Keune WJ, Hipgrave Ederveen AL, van Zeijl L, Joosten RP, Perrakis A J Struct Biol. 2016 Jun 4. pii: S1047-8477(16)30108-3. doi:, 10.1016/j.jsb.2016.06.002. PMID:27268273<ref>PMID:27268273</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5ijs" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 15:37, 23 June 2016
Crystal structure of autotaxin with orthovanadate bound as a trigonal bipyramidal intermediate analog
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