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3nvz

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Revision as of 11:49, 4 August 2016

Crystal Structure of Bovine Xanthine Oxidase in Complex with Indole-3-Aldehyde

Structural highlights

3nvz is a 6 chain structure with sequence from Bos taurus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, , , ,
Related:	3nvv, 3nvw, 3nvy
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[XDH_BOVIN] Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species.

Publication Abstract from PubMed

Xanthine oxidase is a molybdenum-containing hydroxylase that catalyzes the hydroxylation of sp(2)-hybridized carbon centers in a variety of aromatic heterocycles as well as aldehydes. Crystal structures of the oxidase form of the bovine enzyme in complex with a poor substrate indole-3-acetaldehyde and the nonsubstrate guanine have been determined, both at a resolution of 1.6 A. In each structure, a specific and unambiguous orientation of the substrate in the active site is observed in which the hydroxylatable site is oriented away from the active site molybdenum center. The orientation seen with indole-3-acetaldehyde has the substrate positioned with the indole ring rather than the exocyclic aldehyde nearest the molybdenum center, indicating that the substrate must rotate some 30 degrees in the enzyme active site to permit hydroxylation of the aldehyde group (as observed experimentally), accounting for the reduced reactivity of the enzyme toward this substrate. The principal product of hydroxylation of indole-3-acetaldehyde by the bovine enzyme is confirmed to be indole-3-carboxylic acid based on its characteristic UV-vis spectrum, and the kinetics of enzyme reduction are reported. With guanine, the dominant orientation seen crystallographically has the C-8 position that might be hydroxylated pointed away from the active site molybdenum center, in a configuration resembling that seen previously with hypoxanthine (a substrate that is effectively hydroxylated at position 2). The approximately 180 degrees reorientation required to permit reaction is sterically prohibited, indicating that substrate (mis)orientation in the active site is a major factor precluding formation of the highly mutagenic 8-hydroxyguanine.

Substrate orientation and specificity in xanthine oxidase: crystal structures of the enzyme in complex with indole-3-acetaldehyde and guanine.,Cao H, Hall J, Hille R Biochemistry. 2014 Jan 28;53(3):533-41. doi: 10.1021/bi401465u. Epub 2014 Jan 15. PMID:24397336^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Cao H, Hall J, Hille R. Substrate orientation and specificity in xanthine oxidase: crystal structures of the enzyme in complex with indole-3-acetaldehyde and guanine. Biochemistry. 2014 Jan 28;53(3):533-41. doi: 10.1021/bi401465u. Epub 2014 Jan 15. PMID:24397336 doi:http://dx.doi.org/10.1021/bi401465u

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3nvz"

@@ Line 1: / Line 1: @@
 ==Crystal Structure of Bovine Xanthine Oxidase in Complex with Indole-3-Aldehyde==
 <StructureSection load='3nvz' size='340' side='right' caption='[[3nvz]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=I3A:1H-INDOLE-3-CARBALDEHYDE'>I3A</scene>, <scene name='pdbligand=MOS:DIOXOTHIOMOLYBDENUM(VI)+ION'>MOS</scene>, <scene name='pdbligand=MTE:PHOSPHONIC+ACIDMONO-(2-AMINO-5,6-DIMERCAPTO-4-OXO-3,7,8A,9,10,10A-HEXAHYDRO-4H-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-7-YLMETHYL)ESTER'>MTE</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3nvv|3nvv]], [[3nvw|3nvw]], [[3nvy|3nvy]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nvz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nvz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3nvz RCSB], [http://www.ebi.ac.uk/pdbsum/3nvz PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nvz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nvz OCA], [http://pdbe.org/3nvz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3nvz RCSB], [http://www.ebi.ac.uk/pdbsum/3nvz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3nvz ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3nvz" style="background-color:#fffaf0;"></div>
 ==See Also==

3nvz

From Proteopedia

Revision as of 11:49, 4 August 2016

Crystal Structure of Bovine Xanthine Oxidase in Complex with Indole-3-Aldehyde

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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