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4gcz

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Revision as of 14:22, 4 August 2016

Structure of a blue-light photoreceptor

Structural highlights

4gcz is a 2 chain structure with sequence from Bacsu. The March 2015 RCSB PDB Molecule of the Month feature on Phototropin by David Goodsell is 10.2210/rcsb_pdb/mom_2015_3. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
NonStd Res:
Related:	2pr5
Gene:	pfyP, ytvA, BSU30340, fixL, bll2760 (BACSU)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[PHOT_BACSU] Exhibits the same spectroscopical features and blue-light induced photochemistry as plants phototropins, with the reversible formation of a blue-shifted photoproduct, assigned to an FMN-cysteine thiol adduct. Although it is a positive regulator in the activation of the environmental signaling branch of the general stress transcription factor sigma-B, its precise role is undetermined.^[1]

Publication Abstract from PubMed

Two-component systems (TCSs), which comprise sensor histidine kinases (SHK) and response-regulator proteins, represent the predominant strategy by which prokaryotes sense and respond to a changing environment. Despite paramount biological importance, a dearth exists of intact SHK structures containing both sensor and effector modules. Here, we report the full-length crystal structure of the engineered, dimeric, blue-light-regulated SHK YF1 at 2.3 A resolution, in which two N-terminal light-oxygen-voltage (LOV) photosensors are connected by a coiled coil to the C-terminal effector modules. A second coaxial coiled coil derived from the N-termini of the LOV photosensors and inserted between them crucially modulates light regulation: single mutations within this coiled coil attenuate or even invert the signal response of the TCS. Structural motifs identified in YF1 recur in signal receptors, and the underlying signaling principles and mechanisms may be widely shared between soluble and transmembrane, prokaryotic, and eukaryotic signal receptors of diverse biological activity.

Full-length structure of a sensor histidine kinase pinpoints coaxial coiled coils as signal transducers and modulators.,Diensthuber RP, Bommer M, Gleichmann T, Moglich A Structure. 2013 Jul 2;21(7):1127-36. doi: 10.1016/j.str.2013.04.024. Epub 2013, Jun 6. PMID:23746806^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Akbar S, Gaidenko TA, Kang CM, O'Reilly M, Devine KM, Price CW. New family of regulators in the environmental signaling pathway which activates the general stress transcription factor sigma(B) of Bacillus subtilis. J Bacteriol. 2001 Feb;183(4):1329-38. PMID:11157946 doi:10.1128/JB.183.4.1329-1338.2001
↑ Diensthuber RP, Bommer M, Gleichmann T, Moglich A. Full-length structure of a sensor histidine kinase pinpoints coaxial coiled coils as signal transducers and modulators. Structure. 2013 Jul 2;21(7):1127-36. doi: 10.1016/j.str.2013.04.024. Epub 2013, Jun 6. PMID:23746806 doi:10.1016/j.str.2013.04.024

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4gcz"

@@ Line 1: / Line 1: @@
 ==Structure of a blue-light photoreceptor==
 <StructureSection load='4gcz' size='340' side='right' caption='[[4gcz]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4gcz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GCZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GCZ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4gcz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. The March 2015 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Phototropin''  by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2015_3 10.2210/rcsb_pdb/mom_2015_3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GCZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GCZ FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2pr5|2pr5]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pfyP, ytvA, BSU30340, fixL, bll2760 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gcz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gcz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gcz RCSB], [http://www.ebi.ac.uk/pdbsum/4gcz PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gcz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gcz OCA], [http://pdbe.org/4gcz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4gcz RCSB], [http://www.ebi.ac.uk/pdbsum/4gcz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4gcz ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 19: / Line 20: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4gcz" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
@@ Line 24: / Line 26: @@
 </StructureSection>
 [[Category: Bacsu]]
+[[Category: Phototropin]]
+[[Category: RCSB PDB Molecule of the Month]]
 [[Category: Bommer, M]]
 [[Category: Diensthuber, R P]]

4gcz

From Proteopedia

Revision as of 14:22, 4 August 2016

Structure of a blue-light photoreceptor

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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