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4c0b

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Revision as of 14:28, 4 August 2016

Structure of wild-type Clp1p-Pcf11p (454 -563) complex

Structural highlights

4c0b is a 4 chain structure with sequence from Atcc 18824. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	4c0h
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CLP1_YEAST] Component of the cleavage factor IA (CFIA) complex, which is involved in the endonucleolytic cleavage during polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with the cleavage factor NAB4/CFIB and the cleavage and polyadenylation factor (CPF) complex.^[1]

Publication Abstract from PubMed

Pcf11p and Clp1p form a heterodimer and are subunits of the Cleavage Factor IA (CF IA), a complex that is involved in the maturation of the 3'-end of mRNAs in Saccharomyces cerevisiae. The role of Clp1p protein in polyadenylation remains elusive, as does the need for ATP binding by Clp1p. In order to obtain structural details at atomic resolution of point mutants of Clp1p, we solved the crystal structure of Clp1-1p (G135R) point mutant complexed with Pcf11p (454-563) domain. The Clp1-1p-Pcf11p structure provides the atomic details for ATP loss while the point mutation preserves intact the Pcf11p interaction surface of Clp1p. This provides a rationale for the absence of phenotype in the yeast clp1-1 strain. Additionally, the structure allows for the description of an extended binding interface of Pcf11p with Clp1p which is likely to be S. cerevisiae specific.

Structural basis for ATP loss by Clp1p in a G135R mutant protein.,Dupin AF, Fribourg S Biochimie. 2014 Feb 5. pii: S0300-9084(14)00035-2. doi:, 10.1016/j.biochi.2014.01.017. PMID:24508575^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Gross S, Moore C. Five subunits are required for reconstitution of the cleavage and polyadenylation activities of Saccharomyces cerevisiae cleavage factor I. Proc Natl Acad Sci U S A. 2001 May 22;98(11):6080-5. Epub 2001 May 8. PMID:11344258 doi:http://dx.doi.org/10.1073/pnas.101046598
↑ Dupin AF, Fribourg S. Structural basis for ATP loss by Clp1p in a G135R mutant protein. Biochimie. 2014 Feb 5. pii: S0300-9084(14)00035-2. doi:, 10.1016/j.biochi.2014.01.017. PMID:24508575 doi:http://dx.doi.org/10.1016/j.biochi.2014.01.017

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4c0b"

Categories: Atcc 18824 | Dupin, A F | Fribourg, S | 3'-end mrna processing | Transcription

@@ Line 1: / Line 1: @@
 ==Structure of wild-type Clp1p-Pcf11p (454 -563) complex==
 <StructureSection load='4c0b' size='340' side='right' caption='[[4c0b]], [[Resolution|resolution]] 2.77&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4c0h|4c0h]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4c0b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c0b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4c0b RCSB], [http://www.ebi.ac.uk/pdbsum/4c0b PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4c0b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c0b OCA], [http://pdbe.org/4c0b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4c0b RCSB], [http://www.ebi.ac.uk/pdbsum/4c0b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4c0b ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4c0b" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

4c0b

From Proteopedia

Revision as of 14:28, 4 August 2016

Structure of wild-type Clp1p-Pcf11p (454 -563) complex

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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