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3u4l

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Revision as of 18:14, 4 August 2016

Cryocooled bovine profilin:actin crystal structure to 2.4 A

Structural highlights

3u4l is a 2 chain structure with sequence from Bos taurus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
NonStd Res:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ACTB_BOVIN] Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. [PROF1_BOVIN] Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR (By similarity).

Publication Abstract from PubMed

Actin is a ubiquitous eukaryotic protein that is responsible for cellular scaffolding, motility, and division. The ability of actin to form a helical filament is the driving force behind these cellular activities. Formation of a filament depends on the successful exchange of actin's ADP for ATP. Mammalian profilin is a small actin binding protein that catalyzes the exchange of nucleotide and facilitates the addition of an actin monomer to a growing filament. Here, crystal structures of profilin-actin have been determined to show an actively exchanging ATP. Structural analysis shows how the binding of profilin to the barbed end of actin causes a rotation of the small domain relative to the large domain. This conformational change is propagated to the ATP site and causes a shift in nucleotide loops, which in turn causes a repositioning of Ca(2+) to its canonical position as the cleft closes around ATP. Reversal of the solvent exposure of Trp356 is also involved in cleft closure. In addition, secondary calcium binding sites were identified.

Structural basis for profilin-mediated actin nucleotide exchange.,Porta JC, Borgstahl GE J Mol Biol. 2012 Apr 20;418(1-2):103-16. Epub 2012 Feb 22. PMID:22366544^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Porta JC, Borgstahl GE. Structural basis for profilin-mediated actin nucleotide exchange. J Mol Biol. 2012 Apr 20;418(1-2):103-16. Epub 2012 Feb 22. PMID:22366544 doi:10.1016/j.jmb.2012.02.012

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3u4l"

Categories: Bos taurus | Borgstahl, G E | Porta, J C | Atpase | Structural protein

@@ Line 1: / Line 1: @@
 ==Cryocooled bovine profilin:actin crystal structure to 2.4 A==
 <StructureSection load='3u4l' size='340' side='right' caption='[[3u4l]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3u4l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u4l OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3u4l RCSB], [http://www.ebi.ac.uk/pdbsum/3u4l PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3u4l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u4l OCA], [http://pdbe.org/3u4l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3u4l RCSB], [http://www.ebi.ac.uk/pdbsum/3u4l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3u4l ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3u4l" style="background-color:#fffaf0;"></div>
 ==See Also==
 *[[Actin|Actin]]
+*[[Profilin|Profilin]]
 == References ==
 <references/>

3u4l

From Proteopedia

Revision as of 18:14, 4 August 2016

Cryocooled bovine profilin:actin crystal structure to 2.4 A

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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