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4igv

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Revision as of 19:37, 4 August 2016

Crystal structure of kirola (Act d 11)

Structural highlights

4igv is a 1 chain structure with sequence from Actinidia deliciosa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	4igw, 4igx, 4igy, 4ih0, 4ih2, 4ihr
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

The allergen Act d 11, also known as kirola, is a 17kDa protein expressed in large amounts in ripe green and yellow-fleshed kiwifruit. Ten percent of all kiwifruit-allergic individuals produce IgE specific for the protein. Using X-ray crystallography, we determined the first three-dimensional structures of Act d 11, produced from both recombinant expression in Escherichia coli and from the natural source (kiwifruit). While Act d 11 is immunologically correlated with the birch pollen allergen Bet v 1 and other members of the pathogenesis-related protein family 10 (PR-10), it has low sequence similarity to PR-10 proteins. By sequence Act d 11 appears instead to belong to the major latex/ripening-related (MLP/RRP) family, but analysis of the crystal structures shows that Act d 11 has a fold very similar to that of Bet v 1 and other PR-10 related allergens regardless of the low sequence identity. The structures of both the natural and recombinant protein include an unidentified ligand, which is relatively small (about 250Da by mass spectrometry experiments) and most likely contains an aromatic ring. The ligand-binding cavity in Act d 11 is also significantly smaller than those in PR-10 proteins. The binding of the ligand, which we were not able to unambiguously identify, results in conformational changes in the protein that may have physiological and immunological implications. Interestingly, the residue corresponding to Glu45 in Bet v 1 (Glu46), which is important for IgE binding to the birch pollen allergen, is conserved in Act d 11, even though it is not in other allergens with significantly higher sequence identity to Bet v 1. We suggest that the so-called Gly-rich loop (or P-loop), which is conserved in all PR-10 allergens, may be responsible for IgE cross-reactivity between Bet v 1 and Act d 11.

Structural and bioinformatic analysis of the kiwifruit allergen Act d 11, a member of the family of ripening-related proteins.,Chruszcz M, Ciardiello MA, Osinski T, Majorek KA, Giangrieco I, Font J, Breiteneder H, Thalassinos K, Minor W Mol Immunol. 2013 Aug 19;56(4):794-803. doi: 10.1016/j.molimm.2013.07.004. PMID:23969108^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Chruszcz M, Ciardiello MA, Osinski T, Majorek KA, Giangrieco I, Font J, Breiteneder H, Thalassinos K, Minor W. Structural and bioinformatic analysis of the kiwifruit allergen Act d 11, a member of the family of ripening-related proteins. Mol Immunol. 2013 Aug 19;56(4):794-803. doi: 10.1016/j.molimm.2013.07.004. PMID:23969108 doi:10.1016/j.molimm.2013.07.004

1 Structural highlights
2 Publication Abstract from PubMed
3 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4igv"

@@ Line 1: / Line 1: @@
 ==Crystal structure of kirola (Act d 11)==
 <StructureSection load='4igv' size='340' side='right' caption='[[4igv]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=UNL:UNKNOWN+LIGAND'>UNL</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4igw|4igw]], [[4igx|4igx]], [[4igy|4igy]], [[4ih0|4ih0]], [[4ih2|4ih2]], [[4ihr|4ihr]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4igv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4igv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4igv RCSB], [http://www.ebi.ac.uk/pdbsum/4igv PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4igv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4igv OCA], [http://pdbe.org/4igv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4igv RCSB], [http://www.ebi.ac.uk/pdbsum/4igv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4igv ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">
@@ Line 15: / Line 16: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4igv" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

4igv

From Proteopedia

Revision as of 19:37, 4 August 2016

Crystal structure of kirola (Act d 11)

Structural highlights

Publication Abstract from PubMed

References

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