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4chu

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Revision as of 20:50, 4 August 2016

E. coli IscR-DNA complex

Structural highlights

4chu is a 4 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	4cic
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[C3SZN7_ECOLX] Regulates the transcription of several operons and genes involved in the biogenesis of Fe-S clusters and Fe-S-containing proteins (By similarity).[HAMAP-Rule:MF_01176]

Publication Abstract from PubMed

Iron-sulfur clusters function as cofactors of a wide range of proteins, with diverse molecular roles in both prokaryotic and eukaryotic cells. Dedicated machineries assemble the clusters and deliver them to the final acceptor molecules in a tightly regulated process. In the prototypical Gram-negative bacterium Escherichia coli, the two existing iron-sulfur cluster assembly systems, iron-sulfur cluster (ISC) and sulfur assimilation (SUF) pathways, are closely interconnected. The ISC pathway regulator, IscR, is a transcription factor of the helix-turn-helix type that can coordinate a [2Fe-2S] cluster. Redox conditions and iron or sulfur availability modulate the ligation status of the labile IscR cluster, which in turn determines a switch in DNA sequence specificity of the regulator: cluster-containing IscR can bind to a family of gene promoters (type-1) whereas the clusterless form recognizes only a second group of sequences (type-2). However, iron-sulfur cluster biogenesis in Gram-positive bacteria is not so well characterized, and most organisms of this group display only one of the iron-sulfur cluster assembly systems. A notable exception is the unique Gram-positive dissimilatory metal reducing bacterium Thermincola potens, where genes from both systems could be identified, albeit with a diverging organization from that of Gram-negative bacteria. We demonstrated that one of these genes encodes a functional IscR homolog and is likely involved in the regulation of iron-sulfur cluster biogenesis in T. potens. Structural and biochemical characterization of T. potens and E. coli IscR revealed a strikingly similar architecture and unveiled an unforeseen conservation of the unique mechanism of sequence discrimination characteristic of this distinctive group of transcription regulators.

The unique regulation of iron-sulfur cluster biogenesis in a Gram-positive bacterium.,Santos JA, Alonso-Garcia N, Macedo-Ribeiro S, Pereira PJ Proc Natl Acad Sci U S A. 2014 May 20. pii: 201322728. PMID:24847070^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Santos JA, Alonso-Garcia N, Macedo-Ribeiro S, Pereira PJ. The unique regulation of iron-sulfur cluster biogenesis in a Gram-positive bacterium. Proc Natl Acad Sci U S A. 2014 May 20. pii: 201322728. PMID:24847070 doi:http://dx.doi.org/10.1073/pnas.1322728111

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4chu"

@@ Line 1: / Line 1: @@
 ==E. coli IscR-DNA complex==
 <StructureSection load='4chu' size='340' side='right' caption='[[4chu]], [[Resolution|resolution]] 2.49&Aring;' scene=''>
@@ Line 4: / Line 5: @@
 <table><tr><td colspan='2'>[[4chu]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CHU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CHU FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4cic|4cic]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4chu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4chu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4chu RCSB], [http://www.ebi.ac.uk/pdbsum/4chu PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4chu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4chu OCA], [http://pdbe.org/4chu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4chu RCSB], [http://www.ebi.ac.uk/pdbsum/4chu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4chu ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 16: / Line 17: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4chu" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

4chu

From Proteopedia

Revision as of 20:50, 4 August 2016

E. coli IscR-DNA complex

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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